Ramulus mori polysaccharide-loaded PLGA nanoparticles and their anti-inflammatory effects in vivo.

Abstract

In this study, ramulus mori polysaccharide (RMP) was encapsulated into Poly (lactic-co-glycolicacid) (PLGA) to form PLGA-RMP (PR). The aim of study is to investigate anti-inflammatory effects of PR. The particle size of PR nanoparticles was approximately 205.6 ± 1.86 nm. PR nanoparticles showed significant therapeutic effects on colitis mice model, evidenced by attenuation of the loss of body weight, reduction of the DAI score, and restoration of the colon length. From the histopathological analysis, alleviation of the histopathological damage, less production of IFN-γ and IL-6, and improvement of IL-10 were observed with the treatment of PR. Meanwhile, the treatment of PR not only promoted the expression of ZO-1 and occludin, but also improved the contents of acetate, propionate, and butyrate in the colitis colon. Furthermore, PR extenuated the reduction of the diversity and richness of gut microbiota induced by DSS, and decreased the ratio of Firmicutes to Bacteroidetes while increasing the proportion of Clostridium XIVa, Mucispirillum, and Paraprevotella in the gut microbiota. What's more, PR nanoparticles attenuated the metabolic disorders in the colitis colon induced by DSS. These results indicated that PR nanoparticles could serve as a potent nanomedicine to treat IBD and be used as potential prebiotics.

• Authors: Zian Feng, Song Peng, Zhiyong Wu, Lina Jiao, Shuwen Xu, Yu Wu, Zhenguang Liu, Yuanliang Hu, Jiaguo Liu, Yi Wu, Deyun Wang
• Journal: International journal of biological macromolecules (Int J Biol Macromol) Vol. 182 Pg. 2024-2036 (Jul 01 2021) ISSN: 1879-0003 [Electronic] Netherlands
• PMID: 34087293 (Publication Type: Journal Article)
• Copyright: Copyright © 2021 Elsevier B.V. All rights reserved.

Chemical References

• Anti-Inflammatory Agents
• Cytokines
• Fatty Acids
• Polysaccharides
• Tight Junction Proteins
• Polylactic Acid-Polyglycolic Acid Copolymer
• Dextran Sulfate

Topics

• Animals
• Anti-Inflammatory Agents (pharmacology, therapeutic use)
• Colitis (chemically induced, drug therapy, microbiology, physiopathology)
• Colon (drug effects, pathology)
• Cytokines (metabolism)
• Dextran Sulfate
• Disease Models, Animal
• Fatty Acids (metabolism)
• Gastrointestinal Microbiome (drug effects)
• Inflammatory Bowel Diseases (drug therapy, pathology, physiopathology)
• Mice, Inbred C57BL
• Morus (chemistry)
• Nanoparticles (chemistry, ultrastructure)
• Particle Size
• Polylactic Acid-Polyglycolic Acid Copolymer (chemistry)
• Polysaccharides (pharmacology, therapeutic use)
• Static Electricity
• Tight Junction Proteins (metabolism)
• Tight Junctions (drug effects, pathology)
• Mice