Visualizing Tumors in Real Time: A Highly Sensitive PSMA Probe for NIR-II Imaging and Intraoperative Tumor Resection.

Abstract

Owing to the complex anatomical structure, precise resection of a tumor while maintaining adjacent tissue is a challenge in radical prostatectomy for prostate cancer (PCa). Optical imaging in near-infrared window II (NIR-II) is a promising technology for intraoperative guidance, whereas there is no available probe for PCa yet. In this article, a novel probe (PSMA-1092) bearing two prostate-specific membrane antigen (PSMA) binding motifs was developed, displaying excellent optical properties (λmax = 1092 nm) and ultrahigh affinity (Ki = 80 pM) toward PSMA. The tumor was visualized with high resolution (tissue-to-normal tissue ratio = 7.62 ± 1.05) and clear margin by NIR-II imaging using PSMA-1092 in a mouse model. During the tumor resection, residual tumors missed by visible inspection were detected by the real-time imaging. Overall, PSMA-1092 displayed excellent performance in delineating the tumor margin and detecting residual tumors, demonstrating promising potential for precise PCa tumor resection in clinical practice.

• Authors: Longfei Zhang, Xiaojing Shi, Yuying Li, Xiaojiang Duan, Zeyu Zhang, Hualong Fu, Xing Yang, Jie Tian, Zhenhua Hu, Mengchao Cui
• Journal: Journal of medicinal chemistry (J Med Chem) Vol. 64 Issue 11 Pg. 7735-7745 (06 10 2021) ISSN: 1520-4804 [Electronic] United States
• PMID: 34047189 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Fluorescent Dyes
• Prostate-Specific Antigen

Topics

• Animals
• Cell Line, Tumor
• Cell Survival (drug effects)
• Disease Models, Animal
• Drug Stability
• Fluorescent Dyes (chemistry, metabolism, pharmacology)
• Humans
• Hydrogen-Ion Concentration
• Male
• Mice
• Mice, Nude
• Prostate-Specific Antigen (chemistry, metabolism)
• Prostatectomy
• Prostatic Neoplasms (diagnostic imaging, surgery)
• Spectroscopy, Near-Infrared
• Transplantation, Heterologous