[Identification of TCF3-ZNF384 fusion by transcriptome sequencing in B cell acute lymphoblastic leukemia and its laboratory and clinical characteristics].

Abstract

OBJECTIVE:

To detect fusion gene with pathological significance in a patient with refractory and relapsed acute B cell lymphoblastic leukemia (B-ALL) and to explore its laboratory and clinical characteristics.

METHODS:

Transcriptome sequencing was used to detect potential fusion transcripts. Other laboratory results and clinical data of the patient were also analyzed.

RESULTS:

The patient was found to harbor TCF3 exon 17-ZNF384 exon 7 in-frame fusion transcript. The minimal residual disease (MRD) has remained positive after multiple chemotherapy protocols including CD19-, CD22- targeted chimeric antigen receptor T cells immunotherapy. The patient eventually achieved complete remission and sustained MRD negativity after allogeneic hemopoietic stem cell transplantation (allo-HSCT).

CONCLUSION:

Transcriptome sequencing can effectively detect potential fusion genes with clinical significance in leukemia. TCF3-ZNF384 positive B-ALL has unique laboratory and clinical characteristics, may not well respond to chemotherapy and immunotherapy, and is more likely to relapse. Timely allo-HSCT treatment may help such patients to achieve long-term disease-free survival. TCF3-ZNF384 positive B-ALL is not uncommon in pediatric patients but has not been effectively identified.

• Authors: Qisheng Wu, Fang Wang, Junfang Yang, Xue Chen, Xiaoli Ma, Panxiang Cao, Yang Zhang, Daijing Nie, Jiaqi Chen, Xiaosu Zhou, Jiancheng Fang, Mingyue Liu, Min Zhang, Ping Wu, Tong Wang, Hongxing Liu
• Journal: Zhonghua yi xue yi chuan xue za zhi = Zhonghua yixue yichuanxue zazhi = Chinese journal of medical genetics (Zhonghua Yi Xue Yi Chuan Xue Za Zhi) Vol. 38 Issue 4 Pg. 351-354 (Apr 10 2021) ISSN: 1003-9406 [Print] China
• PMID: 33834463 (Publication Type: Journal Article)

Chemical References

• Basic Helix-Loop-Helix Transcription Factors
• TCF3 protein, human
• Trans-Activators
• ZNF384 protein, human

Topics

• B-Lymphocytes
• Basic Helix-Loop-Helix Transcription Factors (genetics)
• Child
• Hematopoietic Stem Cell Transplantation
• Humans
• Laboratories
• Precursor Cell Lymphoblastic Leukemia-Lymphoma (genetics, therapy)
• Trans-Activators (genetics)
• Transcriptome