Efficient induction of neural progenitor cells from human ESC/iPSCs on Type I Collagen.
|
| Abstract |
A stable, rapid and effective neural differentiation method is essential for the clinical applications of human embryonic stem cells (ESCs) or induced pluripotent stem cells (iPSCs) in treating neurological disorders and diseases. Herein, we established a novel and robust monolayer differentiation method to produce functional neural progenitor cells (NPCs) from human ESC/iPSCs on Type I Collagen. The derived cells not only displayed the requisite markers, but also behaved similarly to classic NPCs both in vitro and in vivo. Upon transplantation into traumatic brain injury model, the derived NPCs facilitated recovery from injury. We also found that SMAD signaling stayed down throughout the differentiation process on Type I Collagen, and the pluripotent signals were rapidly downregulated along with raising up of neural early markers on the third day. Meanwhile, ATAC-seq data showed the related mediation of distinct transcriptome and global chromatin dynamics during NPC induction. Totally, our results thus provide a convenient way to generate NPCs from human ESC/iPSCs for neural diseases' treatment.
|
|---|---|
| Authors | Pengfei Liu, Shubin Chen, Yaofeng Wang, Xiaoming Chen, Yiping Guo, Chunhua Liu, Haitao Wang, Yifan Zhao, Di Wu, Yongli Shan, Jian Zhang, Chuman Wu, Dongwei Li, Yanmei Zhang, Tiancheng Zhou, Yaoyu Chen, Xiaobo Liu, Chenxu Li, Lihui Wang, Bei Jia, Jie Liu, Bo Feng, Jinglei Cai, Duanqing Pei |
| Journal | Science China. Life sciences (Sci China Life Sci) Vol. 64 Issue 12 Pg. 2100-2113 (12 2021) ISSN: 1869-1889 [Electronic] China |
| PMID | 33740188 (Publication Type: Journal Article) |
| Copyright | © 2021. Science China Press and Springer-Verlag GmbH Germany, part of Springer Nature. |
| Chemical References |
|
| Topics |
|
Join CureHunter, for free Research Interface BASIC access!
Realize the full power of the drug-disease research graph!