Sterols and triterpenoids from Ganoderma lucidum and their reversal activities of tumor multidrug resistance.

Abstract

Two sterols and seven triterpenoids were isolated and identified from Ganoderma lucidum by silica gel column chromatography, preparative high-performance liquid chromatography and spectra analysis. Then, the multidrug resistance reversal activities of these compounds were assessed using MTT assay. Among these compounds, ganoderol B (3), ganoderone A (4), ganodermanondiol (6) and ganoderiol F (8) were shown to reverse the resistance of human oral epidermoid carcinoma cell line KBv200 to doxorubicin, and the reversal folds were 6.59, 4.70, 4.01 and 7.09, respectively. Ganoderiol F could increase the intracellular accumulation of doxorubicin in KBv200 cells through inhibiting P-glycoprotein transport function. Further mechanistic investigation found that ganoderiol F did not alter P-glycoprotein expression. In conclusion, ganoderiol F has potent effect in reversing P-glycoprotein mediated tumor multidrug resistance. Potential reversal agents against multidrug resistance in tumor may be found in triterpenoids from Ganoderma lucidum.[Formula: see text].

• Authors: Min Wu, Cui-E Shen, Qiao-Fa Lin, Jin-Yi Zhong, Yan-Fei Zhou, Ben-Chen Liu, Jian-Hua Xu, Zhi-Qiang Zhang, Peng Li
• Journal: Natural product research (Nat Prod Res) Vol. 36 Issue 5 Pg. 1396-1399 (Mar 2022) ISSN: 1478-6427 [Electronic] England
• PMID: 33691545 (Publication Type: Journal Article)

Chemical References

• Sterols
• Triterpenes

Topics

• Cell Line, Tumor
• Drug Resistance, Multiple
• Reishi (chemistry)
• Sterols (pharmacology)
• Triterpenes (chemistry, pharmacology)