PGMD/curcumin nanoparticles for the treatment of breast cancer.

Abstract	The present study aims at developing PGMD (poly-glycerol-malic acid-dodecanedioic acid)/curcumin nanoparticles based formulation for anticancer activity against breast cancer cells. The nanoparticles were prepared using both the variants of PGMD polymer (PGMD 7:3 and PGMD 6:4) with curcumin (i.e. CUR NP 7:3 and CUR NP 6:4). The size of CUR NP 7:3 and CUR NP 6:4 were found to be ~ 110 and 218 nm with a polydispersity index of 0.174 and 0.36, respectively. Further, the zeta potential of the particles was - 18.9 and - 17.5 mV for CUR NP 7:3 and CUR NP 6:4, respectively. The entrapment efficiency of both the nanoparticles was in the range of 75-81%. In vitro anticancer activity and the scratch assay were conducted on breast cancer cell lines, MCF-7 and MDA-MB-231. The IC50 of the nanoformulations was observed to be 40.2 and 33.6 μM at 48 h for CUR NP 7:3 and CUR NP 6:4, respectively, in MCF-7 cell line; for MDA-MB-231 it was 43.4 and 30.5 μM. Acridine orange/EtBr and DAPI staining assays showed apoptotic features and nuclear anomalies in the treated cells. This was further confirmed by western blot analysis that showed overexpression of caspase 9 indicating curcumin role in apoptosis.
Authors	Mankamna Kumari, Nikita Sharma, Romila Manchanda, Nidhi Gupta, Asad Syed, Ali H Bahkali, Surendra Nimesh
Journal	Scientific reports (Sci Rep) Vol. 11 Issue 1 Pg. 3824 (02 15 2021) ISSN: 2045-2322 [Electronic] England
PMID	33589661 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
Chemical References	Antineoplastic Agents Drug Carriers Malates Polymers poly-glycerol malate co-dodecanedioate Curcumin Glycerol
Topics	Antineoplastic Agents (administration & dosage) Apoptosis (drug effects) Breast Neoplasms (diagnosis, drug therapy, mortality) Cell Line, Tumor Cell Survival (drug effects) Curcumin (administration & dosage) Dose-Response Relationship, Drug Drug Carriers (chemical synthesis, chemistry) Drug Compounding Drug Liberation Female Glycerol (chemical synthesis) Humans Kinetics Malates (chemical synthesis) Nanoparticles (chemistry, ultrastructure) Particle Size Polymers (chemical synthesis) Spectrum Analysis

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