Comprehensive live-cell imaging analysis of cryptotanshinone and synergistic drug-screening effects in various human and canine cancer cell lines.

Abstract

BACKGROUND:

Several studies have highlighted both the extreme anticancer effects of Cryptotanshinone (CT), a Stat3 crippling component from Salvia miltiorrhiza, as well as other STAT3 inhibitors to fight cancer.

METHODS:

Data presented in this experiment incorporates 2 years of in vitro studies applying a comprehensive live-cell drug-screening analysis of human and canine cancer cells exposed to CT at 20 μM concentration, as well as to other drug combinations. As previously observed in other studies, dogs are natural cancer models, given to their similarity in cancer genetics, epidemiology and disease progression compared to humans.

RESULTS:

Results obtained from several types of human and canine cancer cells exposed to CT and varied drug combinations, verified CT efficacy at combating cancer by achieving an extremely high percentage of apoptosis within 24 hours of drug exposure.

CONCLUSIONS:

CT anticancer efficacy in various human and canine cancer cell lines denotes its ability to interact across different biological processes and cancer regulatory cell networks, driving inhibition of cancer cell survival.

• Authors: Michael L Bittner, Rosana Lopes, Jianping Hua, Chao Sima, Aniruddha Datta, Heather Wilson-Robles
• Journal: PloS one (PLoS One) Vol. 16 Issue 2 Pg. e0236074 ( 2021) ISSN: 1932-6203 [Electronic] United States
• PMID: 33544704 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't, Research Support, U.S. Gov't, Non-P.H.S.)

Chemical References

• Phenanthrenes
• STAT3 Transcription Factor
• STAT3 protein, human
• cryptotanshinone

Topics

• Animals
• Apoptosis (drug effects)
• Cell Line, Tumor
• Cell Survival (drug effects)
• Dogs
• Early Detection of Cancer (methods)
• Humans
• Neoplasms (drug therapy, metabolism)
• Phenanthrenes (metabolism, pharmacology)
• STAT3 Transcription Factor (antagonists & inhibitors)
• Salvia miltiorrhiza (metabolism)
• Signal Transduction (drug effects)