Abstract | INTRODUCTIO: Healing potential of plants is an age-old idea that has recently attained renewed interest. Considering the ineffectiveness, potentially harmful effects, and safety concerns of commonly used synthetic irrigants, the herbal alternatives for endodontic usage might prove to be advantageous. AIM: MATERIALS AND METHODS: The study was conducted in two parts: the first part of the study was cytotoxicity assessment studied using Alamar blue assay. L929 mouse fibroblasts were seeded in 96-well plates at a density of 5000 cells/well and treated with different concentrations of triphala and NaOCl for a period of 24 and 48 h. The percentage of cell viability was then quantified using an Alamar blue assay. The optical density was measured at 570 nm and compared with 620 nm, which was considered as a reference wavelength. The second part of the study was smear layer assessment at the coronal, middle, and apical third of twenty human premolar teeth using scanning electron microscope. RESULTS: CONCLUSION:
Triphala can be considered as a superior irrigant with good antibacterial efficacy and least cytotoxicity potential compared to conventional hypochlorite irrigating agent and provide adequate clearing of smear layer in the coronal and middle third, and further studies are warranted to alter the properties of liquid to make it more cleansable in the apical third of the root.
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Authors | V P Reshma Raj, R Balagopal Varma, J Sureshkumar, Parvathy Kumaran, Arun Mamachan Xavier, Medhini Madhavan |
Journal | Journal of the Indian Society of Pedodontics and Preventive Dentistry
(J Indian Soc Pedod Prev Dent)
2020 Oct-Dec
Vol. 38
Issue 4
Pg. 343-349
ISSN: 1998-3905 [Electronic] India |
PMID | 33402615
(Publication Type: Journal Article)
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Chemical References |
- Plant Extracts
- Root Canal Irrigants
- triphala
- Edetic Acid
- Sodium Hypochlorite
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Topics |
- Dental Pulp Cavity
- Dentin
- Edetic Acid
- Humans
- Microscopy, Electron, Scanning
- Plant Extracts
- Root Canal Irrigants
(toxicity)
- Root Canal Preparation
- Smear Layer
- Sodium Hypochlorite
(toxicity)
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