Abstract |
Immunofluorescent studies in human prostatic carcinoma cells (DU 145) and cultured squirrel fish epithelial cells (a non- cancer cell) revealed that estramustine, a conjugate of estradiol and nor-nitrogen mustard, possessed microtubule disassembly properties. Sixty microM estramustine produced disassembly at both the proximal and distal ends of microtubules, producing short pieces of less than 2 microM which were "wavy" and oriented in a random manner. With increased time of drug exposure these short microtubules disappeared, to be accompanied by a gradual disassembly of a small population of longer microtubules (greater than 7-8 microM). In dividing DU 145 cells it was possible to show a different degree of sensitivity of specific microtubule-containing cellular structures. In mitotic figures the asters were most sensitive and disappeared completely following exposure to estramustine. These were followed by the "pole-to-pole" and "chromosomal" fibers. In cytokinesis, the intercellular fibers between daughter cells were comparatively resistant to the drug. Estramustine did not induce disassembly of the vimentin filaments in non-dividing or dividing cells but did cause their collapse around the nucleus or the mitotic apparatus. These data suggest that specific microtubules have differing sensitivity to estramustine.
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Authors | M Wang, K D Tew, M E Stearns |
Journal | Anticancer research
(Anticancer Res)
1987 Nov-Dec
Vol. 7
Issue 6
Pg. 1165-71
ISSN: 0250-7005 [Print] Greece |
PMID | 3327449
(Publication Type: Journal Article, Research Support, U.S. Gov't, P.H.S.)
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Chemical References |
- Nitrogen Mustard Compounds
- Vimentin
- Estramustine
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Topics |
- Cells, Cultured
- Epithelium
(drug effects)
- Estramustine
(therapeutic use)
- Fluorescent Antibody Technique
- Humans
- Interphase
- Male
- Microtubules
(drug effects)
- Nitrogen Mustard Compounds
(therapeutic use)
- Prostatic Neoplasms
(drug therapy)
- Tumor Cells, Cultured
(drug effects)
- Vimentin
(analysis)
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