MiR-513a-3p inhibits EMT mediated by HOXB7 and promotes sensitivity to cisplatin in ovarian cancer cells.

Abstract

OBJECTIVE:

Our aim was to investigate the biological function and mechanism of action of miR-513a-3p in ovarian cancer cells.

MATERIALS AND METHODS:

In this study, qRT-PCR, Western blots, and immunohistochemistry experiments were among the methods used to examine the expression of miR-513a-3p, HOXB7, and related transcripts within ovarian cancer cells. An MTT assay was conducted to evaluate the viability of ovarian cancer cells in the presence of cisplatin. Transwell and wound-healing assays were performed to examine cell migration and invasion. Dual-Luciferase reporter assays were used to evaluate interactions among the aforementioned target genes. In vivo tumorigenesis experiments were conducted to verify biological effects of miR-513a-3p and HOXB7.

RESULTS:

HOXB7 expression was relatively higher and MiR-513a-3p expression was relatively lower in ovarian cancer cells. Down-regulated expression of miR-513a-3p promoted cell movement via its ability to regulate epithelial-mesenchymal transition (EMT). Furthermore, decreased expression of miR-513a-3p resulted in increased sensitivity to cisplatin and resulted in poor prognosis in ovarian cancer patients who had relapsed after treatment with cisplatin. However, HOXB7 reversed the impact of miR-513a-3p in ovarian cancer cells. These results suggested that miR-513a-3p altered EMT mediated by HOXB7 and cisplatin-resistance.

CONCLUSIONS:

MiR-513a-3p plays a critical role in promoting sensitivity to cisplatin and tumorigenesis via targeting HOXB7 in ovarian cancer.

• Authors: Y Chen, X-H Zhao, D-D Zhang, Y Zhao
• Journal: European review for medical and pharmacological sciences (Eur Rev Med Pharmacol Sci) Vol. 24 Issue 20 Pg. 10391-10402 (10 2020) ISSN: 2284-0729 [Electronic] Italy
• PMID: 33155195 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• HOXB7 protein, human
• Homeodomain Proteins
• MIRN513A1 microRNA, human
• MicroRNAs
• Cisplatin

Topics

• Animals
• Cell Proliferation (drug effects)
• Cisplatin (pharmacology)
• Epithelial-Mesenchymal Transition (genetics)
• Female
• Homeodomain Proteins (genetics, metabolism)
• Humans
• Male
• Mice
• Mice, Nude
• MicroRNAs (genetics, metabolism)
• Neoplasms, Experimental (drug therapy, metabolism, pathology)
• Ovarian Neoplasms (drug therapy, metabolism, pathology)
• Tumor Cells, Cultured