A nonspecific lipid transfer protein, StLTP10, mediates resistance to Phytophthora infestans in potato.

Abstract

Nonspecific lipidtransfer proteins (nsLTPs), which are small, cysteine-rich proteins, belong to the pathogenesis-related protein family, and several of them act as positive regulators during plant disease resistance. However, the underlying molecular mechanisms of these proteins in plant immune responses are unclear. In this study, a typical nsLTP gene, StLTP10, was identified and functionally analysed in potato. StLTP10 expression was significantly induced by Phytophthora infestans, which causes late blight in potato, and defence-related phytohormones, including abscisic acid (ABA), salicylic acid, and jasmonic acid. Characterization of StLTP10-overexpressing and knockdown lines indicated that StLTP10 positively regulates plant resistance to P. infestans. This resistance was coupled with enhanced expression of reactive oxygen species scavenging- and defence-related genes. Furthermore, we identified that StLTP10 physically interacts with ABA receptor PYL4 and affects its subcellular localization. These two proteins work together to regulate stomatal closure during pathogen infection. Interestingly, we also found that wound-induced protein kinase interacts with StLTP10 and positively regulates its protein abundance. Taken together, our results provide insight into the role of StLTP10 in resistance to P. infestans and suggest candidates to enhance broad-spectrum resistance to pathogens in potato.

• Authors: Chenchen Wang, Hongjuan Gao, Zhaohui Chu, Changquan Ji, Yang Xu, Weilin Cao, Shumei Zhou, Yunzhi Song, Hongmei Liu, Changxiang Zhu
• Journal: Molecular plant pathology (Mol Plant Pathol) Vol. 22 Issue 1 Pg. 48-63 (01 2021) ISSN: 1364-3703 [Electronic] England
• PMID: 33118686 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
• Copyright: © 2020 The Authors. Molecular Plant Pathology published by British Society for Plant Pathology and John Wiley & Sons Ltd.

Chemical References

• Carrier Proteins
• Plant Growth Regulators
• Plant Proteins
• lipid transfer protein
• Abscisic Acid
• Salicylic Acid

Topics

• Abscisic Acid (metabolism)
• Carrier Proteins (genetics, metabolism)
• Disease Resistance (genetics)
• Phytophthora infestans (physiology)
• Plant Diseases (immunology, parasitology)
• Plant Growth Regulators (metabolism)
• Plant Proteins (genetics, metabolism)
• Plant Stomata (genetics, immunology, parasitology)
• Salicylic Acid (metabolism)
• Solanum tuberosum (genetics, immunology, parasitology)