Assessment of the functional efficacy of root canal treatment with high-frequency waves in rats.

Abstract

The purpose of this study was to develop a high-frequency wave therapy model in rats and to investigate the influence of high-frequency waves on root canal treatment, which may provide a novel strategy for treating apical periodontitis. Root canal treatments with and without high-frequency wave irradiation were performed on the mandibular first molars of 10-week-old male Wistar rats. The mesial roots were evaluated radiologically, bacteriologically, and immunohistochemically. At 3 weeks after root canal treatment, lesion volume had decreased significantly more in the irradiated group than in the non-irradiated group, indicating successful development of the high-frequency therapy model. The use of high-frequency waves provided no additional bactericidal effect after root canal treatment. However, high-frequency wave irradiation was found to promote healing of periapical lesions on the host side through increased expression of fibroblast growth factor 2 and transforming growth factor-β1 and could therefore be useful as an adjuvant nonsurgical treatment for apical periodontitis.

• Authors: Saori Matsui, Naomichi Yoneda, Hazuki Maezono, Katsutaka Kuremoto, Takuya Ishimoto, Takayoshi Nakano, Hiromichi Yumoto, Shigeyuki Ebisu, Yuichiro Noiri, Mikako Hayashi
• Journal: PloS one (PLoS One) Vol. 15 Issue 9 Pg. e0239660 ( 2020) ISSN: 1932-6203 [Electronic] United States
• PMID: 32991601 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• DNA, Bacterial
• Interleukin-1beta
• Transforming Growth Factor beta1
• Fibroblast Growth Factor 2

Topics

• Animals
• Bacteria (genetics, isolation & purification)
• DNA, Bacterial (isolation & purification, metabolism)
• Dental Pulp Cavity (diagnostic imaging, pathology)
• Fibroblast Growth Factor 2 (metabolism)
• Imaging, Three-Dimensional
• Interleukin-1beta (metabolism)
• Male
• Molar (pathology)
• Periapical Periodontitis (microbiology, pathology, therapy)
• Radio Waves
• Rats
• Rats, Wistar
• Root Canal Therapy (methods)
• Transforming Growth Factor beta1 (metabolism)
• X-Ray Microtomography