Abstract |
In this study, we describe the biological function of the phage-encoded protein RNA polymerase alpha subunit cleavage protein (Rac), a predicted Gcn5-related acetyltransferase encoded by phiKMV-like viruses. These phages encode a single-subunit RNA polymerase for transcription of their late (structure- and lysis-associated) genes, whereas the bacterial RNA polymerase is used at the earlier stages of infection. Rac mediates the inactivation of bacterial transcription by introducing a specific cleavage in the α subunit of the bacterial RNA polymerase. This cleavage occurs within the flexible linker sequence and disconnects the C-terminal domain, required for transcription initiation from most highly active cellular promoters. To achieve this, Rac likely taps into a novel post-translational modification (PTM) mechanism within the host Pseudomonas aeruginosa. From an evolutionary perspective, this novel phage-encoded regulation mechanism confirms the importance of PTMs in the prokaryotic metabolism and represents a new way by which phages can hijack the bacterial host metabolism.
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Authors | Pieter-Jan Ceyssens, Jeroen De Smet, Jeroen Wagemans, Natalia Akulenko, Evgeny Klimuk, Subray Hedge, Marleen Voet, Hanne Hendrix, Jan Paeshuyse, Bart Landuyt, Hua Xu, John Blanchard, Konstantin Severinov, Rob Lavigne |
Journal | Viruses
(Viruses)
Vol. 12
Issue 9
(09 02 2020)
ISSN: 1999-4915 [Electronic] Switzerland |
PMID | 32887488
(Publication Type: Journal Article, Research Support, N.I.H., Extramural, Research Support, Non-U.S. Gov't)
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Chemical References |
- Bacterial Proteins
- Viral Proteins
- Acetyltransferases
- DNA-Directed RNA Polymerases
- RNA polymerase alpha subunit
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Topics |
- Acetyltransferases
(genetics, metabolism)
- Bacterial Proteins
(genetics, metabolism)
- DNA-Directed RNA Polymerases
(genetics, metabolism)
- Host-Pathogen Interactions
- Pseudomonas Phages
(enzymology, genetics)
- Pseudomonas aeruginosa
(enzymology, genetics, virology)
- Transcription, Genetic
- Viral Proteins
(genetics, metabolism)
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