Indocyanine Green Labels an Orthotopic Nude-Mouse Model of Very-Early Colon-Cancer Liver Metastases.

Abstract

BACKGROUND/AIM:

Near-infrared (NIR) fluorescence imaging using indocyanine green (ICG) is used to visualize colon-cancer liver metastases (CCLM) during surgery. The present study aimed to use ICG to visualize small CCLM in an orthotopic mouse model.

MATERIALS AND METHODS:

A 3-mm fragment of HT29 human colon cancer was implanted to the liver of 5 athymic nude mice (nu/nu). The Pearl Trilogy Small Animal Fluorescence Imaging system was used 24 h after intravenous (IV) injection of 0.025 mg (0.25 mg/ml) ICG.

RESULTS:

In four of the five mice, tumor fluorescence was detected. Small tumors (approximately 3 mm) were distinctly visualized with a minimal fluorescence liver signal, with a mean tumor to liver signal ratio of 1.81 (SD±0.167).

CONCLUSION:

Small CCLM can be clearly identified by ICG fluorescence in an orthotopic nude-mouse model. This model is translatable to the clinic for the detection of occult early CCLM.

• Authors: Yoshihiko Tashiro, Hannah M Hollandsworth, Hiroto Nishino, Jun Yamamoto, Siamak Amirfakhri, Filemoni Filemoni, Norihiko Sugisawa, Takeshi Aoki, Masahiko Murakami, Robert M Hoffman, Michael Bouvet
• Journal: In vivo (Athens, Greece) (In Vivo) 2020 Sep-Oct Vol. 34 Issue 5 Pg. 2277-2280 ISSN: 1791-7549 [Electronic] Greece
• PMID: 32871750 (Publication Type: Journal Article)
• Copyright: Copyright© 2020, International Institute of Anticancer Research (Dr. George J. Delinasios), All rights reserved.

Chemical References

• Indocyanine Green

Topics

• Animals
• Colon
• Indocyanine Green
• Liver Neoplasms (diagnostic imaging)
• Mice
• Mice, Nude
• Optical Imaging