Abstract | BACKGROUND:
Apramycin is used exclusively for the treatment of Escherichia coli ( E.coli) infections in swine around the world since the early 1980s. Recently, many research papers have demonstrated that apramycin has significant in vitro activity against multidrug-resistant E.coli isolated in hospitals. Therefore, ensuring the proper use of apramycin in veterinary clinics is of great significance of public health. The objectives of this study were to develop a wild-type cutoff for apramycin against E.coli using a statistical method recommended by Clinical and Laboratory Standards Institute (CLSI) and to investigate the prevalence of resistance genes that confer resistance to apramycin in E. coli. RESULTS:
Apramycin susceptibility testing of 1230 E.coli clinical isolates from swine were determinded by broth microdilution testing according to the CLSI document M07-A9. A total number of 310 E.coli strains from different minimum inhibitory concentration (MIC) subsets (0.5-256 μg/mL) were selected for the detection of resistance genes (aac(3)-IV; npmA; apmA) in E. coli by PCR. The percentage of E. coli isolates at each MIC (0.5, 1, 2, 4, 8, 16, 32, 64, 128, and 256 μg/mL) was 0.08, 0.08, 0.16, 2.93, 31.14, 38.86, 12.85, 2.03, 1.46, and 10.41%. The MIC50 and MIC90 were 16 and 64 μg/mL. All the 310 E.coli isolates were negative for npmA and apmA gene, and only the aac(3)-IV gene was detected in this study. CONCLUSIONS: The wild-type cutoff for apramycin against E.coli was defined as 32 μg/mL. The prevelance of aac(3)-IV gene mainly concentrated in these MIC subsets 'MIC ≥ 64 μg/ mL', which indicates that the wild-type cutoff established in our study is reliable. The wild-type cutoff offers interpretion criteria of apramycin susceptibility testing of E.coli.
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Authors | Yuqi Yang, Tianshi Xiao, Jiarui Li, Ping Cheng, Fulei Li, Hongxiao Yu, Ruimeng Liu, Ishfaq Muhammad, Xiuying Zhang |
Journal | BMC veterinary research
(BMC Vet Res)
Vol. 16
Issue 1
Pg. 309
(Aug 26 2020)
ISSN: 1746-6148 [Electronic] England |
PMID | 32847547
(Publication Type: Journal Article)
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Chemical References |
- Anti-Bacterial Agents
- Nebramycin
- apramycin
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Topics |
- Animals
- Anti-Bacterial Agents
(pharmacology)
- Drug Resistance, Bacterial
(genetics)
- Escherichia coli
(drug effects, genetics)
- Escherichia coli Infections
(drug therapy, veterinary)
- Microbial Sensitivity Tests
(veterinary)
- Nebramycin
(analogs & derivatives, pharmacology)
- Swine
- Swine Diseases
(drug therapy, microbiology)
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