Generation of three Duchenne muscular dystrophy patient-derived induced pluripotent stem cell (iPSC) lines ICGi002-A, ICGi002-B and ICGi002-C.

Abstract

Duchenne muscular dystrophy (DMD) is a severe and rapidly progressive hereditary muscular disease with X-linked recessive inheritance, occurring mainly in males. A complete loss of dystrophin resulted from out-of-frame deletion mutations in the DMD gene leads to Duchenne muscular dystrophy. DMD induced pluripotent stem cells (iPSCs) are a suitable cell model to study muscle development and disease mechanisms underlying muscular dystrophy and to screen novel compounds with potential therapeutic effects. We generated iPSCs from a DMD patient using non-integrating episomal plasmid vectors. The obtained iPSC lines showed ESC-like morphology, expression pluripotency markers, displayed a normal karyotype and possessed trilineage differentiation potential.

• Authors: K R Valetdinova, M A Maretina, Y V Vyatkin, M P Perepelkina, A A Egorova, V S Baranov, A V Kiselev, P M Gershovich, S M Zakian
• Journal: Stem cell research (Stem Cell Res) Vol. 48 Pg. 101941 (10 2020) ISSN: 1876-7753 [Electronic] England
• PMID: 32777771 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
• Copyright: Copyright © 2020 The Authors. Published by Elsevier B.V. All rights reserved.

Chemical References

• Dystrophin

Topics

• Cell Differentiation
• Dystrophin (genetics)
• Humans
• Induced Pluripotent Stem Cells
• Male
• Muscular Dystrophy, Duchenne (genetics)