Expanded Poly(tetrafluoroethylene) Blood Vessel Grafts with Embedded Reactive Oxygen Species (ROS)-Responsive Antithrombogenic Drug for Elimination of Thrombosis.

Abstract

Treatment of cardiovascular diseases suffers from the lack of transplantable small-diameter blood vessel (SDBV) grafts that can prohibit/eliminate thrombosis. Although expanded poly(tetrafluoroethylene) (ePTFE) has the potential to be used for SDBV grafts, recurrence of thrombus remains the biggest challenge. In this study, a reactive oxygen species (ROS)-responsive antithrombogenic drug synthesis and a bulk coating process were employed to fabricate functional ePTFE grafts capable of prohibiting/eliminating blood clots. The synthesized drug that would release antiplatelet ethyl salicylate (ESA), in responding to ROS, was dissolved in a polycaprolactone (PCL) solution, followed by a bulk coating of the as-fabricated ePTFE grafts with the PCL/drug solution. Nuclear magnetic resonance (NMR) spectroscopy, Fourier-transform infrared (FTIR) spectroscopy, scanning electron microscopy (SEM), and atomic force microscopy (AFM) were employed to investigate and confirm the synthesis and presence of the ROS-responsive drug in the ePTFE grafts. The ESA release functions were demonstrated via the drug-release profile and dynamic anticoagulation tests. The biocompatibility of the ROS-responsive ePTFE grafts was demonstrated via lactate dehydrogenase (LDH) cytotoxicity assays, live and dead cell assays, cell morphology, and cell-graft interactions. The ROS-responsive, antithrombogenic ePTFE grafts provide a feasible way for maintaining long-term patency, potentially solving a critical challenge in SDBV applications.

• Authors: Dongfang Wang, Yiyang Xu, Lixia Wang, Xiaofeng Wang, Cuihong Ren, Bo Zhang, Qian Li, James A Thomson, Lih-Sheng Turng
• Journal: ACS applied materials & interfaces (ACS Appl Mater Interfaces) Vol. 12 Issue 26 Pg. 29844-29853 (Jul 01 2020) ISSN: 1944-8252 [Electronic] United States
• PMID: 32496045 (Publication Type: Journal Article)

Chemical References

• Fibrinolytic Agents
• Fluorocarbons
• Polyesters
• Polymers
• Reactive Oxygen Species
• Salicylates
• polycaprolactone
• ethyl salicylate
• Polytetrafluoroethylene
• L-Lactate Dehydrogenase
• tetrafluoroethylene

Topics

• Animals
• Fibrinolytic Agents (therapeutic use)
• Fluorocarbons (chemistry)
• Humans
• L-Lactate Dehydrogenase (metabolism)
• Magnetic Resonance Spectroscopy
• Microscopy, Atomic Force
• Polyesters (chemistry)
• Polymers (chemistry)
• Polytetrafluoroethylene (chemistry)
• Reactive Oxygen Species (metabolism)
• Salicylates (chemistry)
• Spectroscopy, Fourier Transform Infrared
• Thrombosis (drug therapy)