Clinical significance of neuropilin-2 expression in laryngeal squamous cell carcinoma.

Abstract

PURPOSE:

The purpose of the study is to evaluate the expression of NRP-2 and explore its role in Laryngeal squamous cell carcinoma (LSCC).

MATERIALS AND METHODS:

NRP-2 expression in 70 primary LSCC tissue specimens were analyzed by immunohistochemistry and correlated with clinicopathological parameters and patients´ survival rate. Additionally, 9 paired LSCC tissues were evaluated for NRP-2 expression by Western blotting.

RESULTS:

The Western blotting indicated that NRP-2 expression levels in LSCC were significantly higher than those in the paraneoplastic tissues (P < 0.05). Immunohistochemistry staining revealed that NRP-2 was detected in all primary tumor samples, moreover, high expression of NRP-2 was significantly correlated with TNM stage (P < 0.05), clinical stage (P < 0.05), histological classification (P < 0.05), lymph node metastasis (P < 0.05) and recurrence (P = 0.001). Survival curves determined by the Kaplan-Meier method showed that high expression of NRP-2 can reduce overall survival (both group P < 0.05). Then we combined the NRP-2 expression and lymph node status, and Kaplan-Meier survival showed patients with high expression of NRP-2 or lymph node metastasis (+) had both shorter disease-free and overall survival than others (both P < 0.05). Multivariate Cox proportional hazards model analysis confirmed that histological grade (P = 0.045), lymph node metastasis (P = 0.020) and high expression of NRP-2 (P = 0.033) were statistically significant, independent predictor of prognosis.

CONCLUSIONS:

NRP-2 may contribute to LSCC progression and represents as a novel prognostic indicator as well as a potential therapeutic target for LSCC.

• Authors: Danhui Yin, Lu Guo, Shisheng Li, Ayinuer Tuerdi, Xinming Yang, Qinglai Tang, Shuang Wang, Jiajia Liu, Peiying Huang, Mengmeng Li
• Journal: American journal of otolaryngology (Am J Otolaryngol) 2020 Jul - Aug Vol. 41 Issue 4 Pg. 102540 ISSN: 1532-818X [Electronic] United States
• PMID: 32480134 (Publication Type: Journal Article)
• Copyright: Copyright © 2020 Elsevier Inc. All rights reserved.

Chemical References

• Neuropilin-2
• neuropilin-2, human

Topics

• Disease Progression
• Female
• Gene Expression
• Gene Expression Regulation, Neoplastic
• Humans
• Laryngeal Neoplasms (genetics, mortality, pathology)
• Lymphatic Metastasis
• Male
• Middle Aged
• Neuropilin-2 (genetics, metabolism)
• Prognosis
• Proportional Hazards Models
• Squamous Cell Carcinoma of Head and Neck (genetics, mortality, pathology)
• Survival Rate