Cancer invasion and
metastasis are the leading causes of death. The process of
metastasis or
tumor cell dissemination is still much of a mystery. Emerging evidence has shown that epithelial-mesenchymal transition (EMT) plays a vital role in the progression of malignant
tumor including the inducing cell invasion and
metastasis as well as promoting drug resistance.
Vinorelbine is a traditional chemotherapeutic agent for treatment of
lung cancer and
breast cancer by the selectivity to mitotic microtubules. The aim of this study was to investigate the effect of
vinorelbine on three metastatic
cancer cells including
lung cancer (H1975),
liver cancer (HepG2), and
colon cancer (HCT116) cells through inhibition of metastatic abilities and EMT program.
Vinorelbine inhibited the
cancer cell proliferation by MTT and colony formation assays and inducing G2/M arrest and cell apoptosis via regulation of Bax, Bcl-2, and Bcl-xL.
Vinorelbine decrease the migration and invasion ability of the
cancer cells by wound healing assay and Tran swell test. The molecular mechanisms of
vinorelbine suppressing the metastatic phenotypes of
cancer cells through modulation of
E-cadherin,
N-cadherin,
vimentin and
transcription factors Snail, MMP-2 and MMP-9. Our results demonstrated that
vinorelbine inhibited the
cancer cell
metastasis through a reduction in metastatic mobility, such as migration, invasion, and the EMT. It provided the evidence that
vinorelbine can be used alone or with other agents for treatment of metastatic
lung cancer,
liver cancer and
colon cancer.