The effect of sphingosine-1-phosphate on the endothelial glycocalyx during ischemia-reperfusion injury in the isolated rat heart.

Abstract

OBJECTIVE:

Sphingosine-1-phosphate is a natural metabolite that is cardioprotective, but its effects on endothelial glycocalyx damage during ischemia-reperfusion are unknown. Therefore, we investigated the effect of sphingosine-1-phosphate on the endothelial glycocalyx during ischemia-reperfusion.

METHODS:

Isolated hearts from Wistar rats were perfused on a Langendorff system with Krebs-Henseleit buffer and pretreated with sphingosine-1-phosphate (10 nmol/L) before ischemia-reperfusion. Infarct size was measured by triphenyl tetrazolium chloride staining (n ≥ 6 per group). Cardiac edema was assessed by calculating total water content (n = 7 per group) and histologically quantifying the interstitial compartment (n ≥ 3 per group). The post-ischemic coronary release of syndecan-1 was quantified using ELISA. Syndecan-1 immunostaining intensity was assessed in perfusion-fixed hearts (n ≥ 3 per group).

RESULTS:

Pretreatment with sphingosine-1-phosphate decreased infarct size in isolated hearts subjected to ischemia-reperfusion (P = .01 vs ischemia-reperfusion). However, sphingosine-1-phosphate had no effect on syndecan-1 levels in the coronary effluent or on the intensity of the syndecan-1 immunostaining signal in cardiac tissue. Heart total water content was not significantly different between control and ischemic groups but was significantly decreased in hearts treated with sphingosine-1-phosphate alone.

CONCLUSION:

These results suggest that sphingosine-1-phosphate-induced cardioprotection against ischemia-reperfusion injury is not mediated by the maintenance of syndecan-1 in the endothelial glycocalyx.

• Authors: Hala Araibi, Elizabeth van der Merwe, Asfree Gwanyanya, Roisin Kelly-Laubscher
• Journal: Microcirculation (New York, N.Y. : 1994) (Microcirculation) Vol. 27 Issue 5 Pg. e12612 (07 2020) ISSN: 1549-8719 [Electronic] United States
• PMID: 32017300 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
• Copyright: © 2020 John Wiley & Sons Ltd.

Chemical References

• Lysophospholipids
• Syndecan-1
• sphingosine 1-phosphate
• Sphingosine

Topics

• Animals
• Endothelium, Vascular (metabolism, pathology)
• Glycocalyx (metabolism, pathology)
• Lysophospholipids (metabolism, pharmacology)
• Male
• Myocardial Reperfusion Injury (metabolism, pathology)
• Myocardium (metabolism, pathology)
• Rats
• Rats, Wistar
• Sphingosine (analogs & derivatives, metabolism, pharmacology)
• Syndecan-1 (metabolism)