Abstract |
Hepatocellular carcinoma (HCC) is a tough opponent. HCC contributes to 14.8% of all cancer mortality in Egypt. There are many choices for management of HCC; however tumor relapse has been reported in animal and clinical studies. This study was conducted to investigate the impact of low dose γ-irradiation (LDR) and combretastatin A-4 disodium phosphate (CA-4DP) on HCC recurrence. HCC was induced in male Wistar albino rats by oral administration of N-nitrosodiethylamine (NDEA) for 17 weeks. We evaluated the expression of the endothelial cell marker (CD31) by immunostaining. Expression of Rho Associated Coiled-Coil Containing Protein Kinase 1(ROCK1) and Vascular endothelial growth factor ( VEGF) expression was assessed by real-time PCR after (6, 24 and 48 h). Our results showed that expression of CD31 and gene expression of ROCK1 and VEGF was significantly repressed at all-time intervals by combination therapy ofLDR and CA-4DP as compared with untreated NDEA/HCC group and NDEA/HCC groups treated with either LDR or CA-4DP alone, (P < 0.05). Our study demonstrated the additive effect of LDR in combination with CA-4DP in suppression of HCC.
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Authors | Zakaria Mohamed Alghzzawy, Tarek Khaled Elmaghraby, Sanaa Abd El-Hamid Hagag, Mohammed Hussein Awwad |
Journal | Molecular biology reports
(Mol Biol Rep)
Vol. 47
Issue 3
Pg. 1883-1893
(Mar 2020)
ISSN: 1573-4978 [Electronic] Netherlands |
PMID | 32016634
(Publication Type: Journal Article)
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Chemical References |
- Antineoplastic Agents, Phytogenic
- Platelet Endothelial Cell Adhesion Molecule-1
- Stilbenes
- Vascular Endothelial Growth Factor A
- vascular endothelial growth factor A, rat
- Diethylnitrosamine
- ROCK1 protein, rat
- rho-Associated Kinases
- fosbretabulin
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Topics |
- Animals
- Antineoplastic Agents, Phytogenic
(administration & dosage, pharmacology)
- Carcinoma, Hepatocellular
(chemically induced, genetics, metabolism, therapy)
- Chemoradiotherapy
- Combined Modality Therapy
- Diethylnitrosamine
(adverse effects)
- Down-Regulation
- Egypt
- Gamma Rays
- Gene Expression Regulation, Neoplastic
(drug effects)
- Liver Neoplasms
(chemically induced, genetics, metabolism, therapy)
- Male
- Platelet Endothelial Cell Adhesion Molecule-1
(metabolism)
- Rats
- Rats, Wistar
- Stilbenes
(administration & dosage, pharmacology)
- Treatment Outcome
- Vascular Endothelial Growth Factor A
(genetics)
- Xenograft Model Antitumor Assays
- rho-Associated Kinases
(genetics)
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