Tannic Acid Promotes TRAIL-Induced Extrinsic Apoptosis by Regulating Mitochondrial ROS in Human Embryonic Carcinoma Cells.

Abstract

: Human embryonic carcinoma (EC; NCCIT) cells have self-renewal ability and pluripotency. Cancer stem cell markers are highly expressed in NCCIT cells, imparting them with the pluripotent nature to differentiate into other cancer types, including breast cancer. As one of the main cancer stem cell pathways, Wnt/β-catenin is also overexpressed in NCCIT cells. Thus, inhibition of these pathways defines the ability of a drug to target cancer stem cells. Tannic acid (TA) is a natural polyphenol present in foods, fruits, and vegetables that has anti-cancer activity. Through Western blotting and PCR, we demonstrate that TA inhibits cancer stem cell markers and the Wnt/β-catenin signaling pathway in NCCIT cells and through a fluorescence-activated cell sorting analysis we demonstrated that TA induces sub-G1 cell cycle arrest and apoptosis. The mechanism underlying this is the induction of mitochondrial reactive oxygen species (ROS) (mROS), which then induce the tumor necrosis factor-related apoptosis-inducing ligand (TRAIL)-mediated extrinsic apoptosis pathway instead of intrinsic mitochondrial apoptosis pathway. Moreover, ribonucleic acid sequencing data with TA in NCCIT cells show an elevation in TRAIL-induced extrinsic apoptosis, which we confirm by Western blotting and real-time PCR. The induction of human TRAIL also proves that TA can induce extrinsic apoptosis in NCCIT cells by regulating mROS.

• Authors: Nipin Sp, Dong Young Kang, Eun Seong Jo, Alexis Rugamba, Wan Seop Kim, Yeong-Min Park, Dae-Yong Hwang, Ji-Seung Yoo, Qing Liu, Kyoung-Jin Jang, Young Mok Yang
• Journal: Cells (Cells) Vol. 9 Issue 2 (01 23 2020) ISSN: 2073-4409 [Electronic] Switzerland
• PMID: 31979292 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Biomarkers, Tumor
• Reactive Oxygen Species
• TNF-Related Apoptosis-Inducing Ligand
• Tannins
• Adenosine Triphosphate

Topics

• Adenosine Triphosphate (metabolism)
• Apoptosis (drug effects)
• Biomarkers, Tumor (metabolism)
• Carcinoma (metabolism, pathology)
• Cell Line, Tumor
• Cell Proliferation (drug effects)
• Down-Regulation (drug effects)
• G1 Phase Cell Cycle Checkpoints (drug effects)
• Humans
• Mitochondria (drug effects, metabolism)
• Models, Biological
• Neoplastic Stem Cells (drug effects, metabolism, pathology)
• Reactive Oxygen Species (metabolism)
• TNF-Related Apoptosis-Inducing Ligand (pharmacology)
• Tannins (pharmacology)
• Wnt Signaling Pathway (drug effects)