Abstract |
The echinocandins anidulafungin (ANID) and micafungin ( MICA) are recommended for treatment of invasive Candida infections. As target-site concentrations of antimicrobial agents are crucial for eradication of pathogens, we established and validated high-performance liquid chromatography-UV detection (HPLC-UV) assays for quantification of ANID and MICA in human plasma, ascites fluid, pleural effusion, and in cerebrospinal fluid (CSF). Sample pre-purification was performed by protein precipitation with acetonitrile followed by solid phase extraction. For both assays, intra- and interday precision, and accuracy fulfilled the requirements for bioanalytical methods issued by the European Medicine Agency (EMA). The lower limit of quantification was 0.01 mg/L for both drugs. At 25 °C, ANID and MICA concentrations declined by up to 70% within 24 h. Concentrations remained stable over 24 h at 4 °C and over four weeks at -80 °C. In conclusion, the developed methods are fit for the assessment of target-site pharmacokinetics of ANID and MICA in clinical studies.
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Authors | René Welte, Herbert Oberacher, Bernhard Schwärzler, Michael Joannidis, Romuald Bellmann |
Journal | Journal of chromatography. B, Analytical technologies in the biomedical and life sciences
(J Chromatogr B Analyt Technol Biomed Life Sci)
Vol. 1139
Pg. 121937
(Feb 15 2020)
ISSN: 1873-376X [Electronic] Netherlands |
PMID | 31958565
(Publication Type: Journal Article)
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Copyright | Copyright © 2019 The Authors. Published by Elsevier B.V. All rights reserved. |
Chemical References |
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Topics |
- Anidulafungin
(analysis, therapeutic use)
- Ascitic Fluid
(chemistry)
- Candidiasis, Invasive
(drug therapy)
- Chromatography, High Pressure Liquid
(methods)
- Drug Monitoring
(methods)
- Humans
- Limit of Detection
- Linear Models
- Micafungin
(analysis, therapeutic use)
- Reproducibility of Results
- Solid Phase Extraction
- Spectrophotometry, Ultraviolet
(methods)
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