Surfactant-Stripped Pheophytin Micelles for Multimodal Tumor Imaging and Photodynamic Therapy.

Abstract

Porphyrin-based nanomaterials can inherently integrate multiple contrast imaging functionalities with phototherapeutic capabilities. We dispersed pheophytin (Pheo) into Pluronic F127 and carried out low-temperature surfactant-stripping to remove the bulk surfactant. Surfactant-stripped Pheo (ss-Pheo) micelles exhibited a similar size, but higher near-infrared fluorescence, compared to two other nanomaterials also with high porphyrin density (surfactant-stripped chlorophyll micelles and porphysomes). Singlet oxygen generation, which was higher for ss-Pheo, enabled photodynamic therapy (PDT). ss-Pheo provided contrast for photoacoustic and fluorescence imaging, and following seamless labeling with 64Cu, was used for positron emission tomography. ss-Pheo had a long blood circulation and favorable accumulation in an orthotopic murine mammary tumor model. Trimodal tumor imaging was demonstrated, and PDT resulted in delayed tumor growth.

• Authors: Dana Moukheiber, Upendra Chitgupi, Kevin A Carter, Dandan Luo, Boyang Sun, Shreya Goel, Carolina A Ferreira, Jonathan W Engle, Depeng Wang, Jumin Geng, Yumiao Zhang, Jun Xia, Weibo Cai, Jonathan F Lovell
• Journal: ACS applied bio materials (ACS Appl Bio Mater) Vol. 2 Issue 1 Pg. 544-554 (Jan 22 2019) ISSN: 2576-6422 [Electronic] United States
• PMID: 31853516 (Publication Type: Journal Article)