Red pepper peptide coatings control Staphylococcus epidermidis adhesion and biofilm formation.

Abstract

Medical devices (indwelling) have greatly improved healthcare. Nevertheless, infections related to the use of these apparatuses continue to be a major clinical concern. Biofilms form on surfaces after bacterial adhesion, and they function as bacterial reservoirs and as resistance and tolerance factors against antibiotics and the host immune response. Technological strategies to control biofilms and bacterial adhesion, such as the use of surface coatings, are being explored more frequently, and natural peptides may promote their development. In this study, we purified and identified antibiofilm peptides from Capsicum baccatum (red pepper) using chromatography-tandem mass spectrometry, MALDI-MS, MS/MS and bioinformatics. These peptides strongly controlled biofilm formation by Staphylococcus epidermidis, the most prevalent pathogen in device-related infections, without any antibiotic activity. Furthermore, natural peptide-coated surfaces dislayed effective antiadhesive proprieties and showed no cytotoxic effects against different representative human cell lines. Finally, we determined the lead peptide predicted by Mascot and identified CSP37, which may be useful as a prime structure for the design of new antibiofilm agents. Together, these results shed light on natural Capsicum peptides as a possible antiadhesive coat to prevent medical device colonization.

• Authors: Rafael Gomes Von Borowski, Muriel Primon Barros, Denise Brentan da Silva, Norberto Peporine Lopes, Karine Rigon Zimmer, Charley Christian Staats, Cristiane Bernardes de Oliveira, Emmanuel Giudice, Reynald Gillet, Alexandre José Macedo, Simone Cristina Baggio Gnoatto, Aline Rigon Zimmer
• Journal: International journal of pharmaceutics (Int J Pharm) Vol. 574 Pg. 118872 (Jan 25 2020) ISSN: 1873-3476 [Electronic] Netherlands
• PMID: 31812797 (Publication Type: Journal Article)
• Copyright: Copyright © 2019 Elsevier B.V. All rights reserved.

Chemical References

• Anti-Bacterial Agents
• Coated Materials, Biocompatible
• Peptides

Topics

• Anti-Bacterial Agents (pharmacology)
• Bacterial Adhesion (drug effects)
• Biofilms (drug effects)
• Capsicum (chemistry)
• Cell Line, Tumor
• Coated Materials, Biocompatible (chemistry)
• HCT116 Cells
• Humans
• MCF-7 Cells
• PC-3 Cells
• Peptides (pharmacology)
• Staphylococcal Infections (drug therapy)
• Staphylococcus epidermidis (drug effects)
• Tandem Mass Spectrometry (methods)