Quercetin alleviates lipopolysaccharide-induced inflammatory responses by up-regulation miR-124 in human renal tubular epithelial cell line HK-2.
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| Abstract |
Chronic kidney disease (CKD) is a persistent kidney structural and functional disorder. Quercetin is one of active extracted flavonoids and has protective effects. Therefore, we proposed to survey the effect of Quercetin on CKD. HK-2 cells were preprocessed by Quercetin and then irritated with lipopolysaccharide (LPS). CCK-8 assay and flow cytometry were utilized to test viability and apoptosis. ELISA assay was utilized to estimate the IL-6 and TNF-α secretion. Western blot was performed to examine the expression of apoptosis and inflammation-associated mediators. After that HK-2 cells were transfected by miR-124 inhibitor. And the above-mentioned parameters were reassessed. LPS stimulated apoptosis and declined viability in HK-2 cells. Additionally, LPS stimulated inflammatory responses. Meanwhile, Quercetin attenuated LPS-stimulated apoptosis, production of IL-6, and TNF-α in experimental cells. Subsequently, MyD88 and miR-124 expression was elevated by LPS and alleviated by Quercetin. Finally, Quercetin exerted its protective function through NF-κB pathway via up-regulating miR-124. Our data demonstrated that Quercetin reduced apoptosis and inflammation stimulated by LPS in HK-2 cells. Moreover, Quercetin alleviated LPS-stimulated injury by up-regulating miR-124.
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| Authors | Shuxia Guo, Juanjuan Sun, Yan Zhuang |
| Journal | BioFactors (Oxford, England) (Biofactors) Vol. 46 Issue 3 Pg. 402-410 (May 2020) ISSN: 1872-8081 [Electronic] Netherlands |
| PMID | 31804760 (Publication Type: Journal Article, Retracted Publication) |
| Copyright | © 2019 International Union of Biochemistry and Molecular Biology. |
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