TCTP from Loxosceles Intermedia (Brown Spider) Venom Contributes to the Allergic and Inflammatory Response of Cutaneous Loxoscelism.

Abstract

LiTCTP is a toxin from the Translationally Controlled Tumor Protein (TCTP) family identified in Loxosceles brown spider venoms. These proteins are known as histamine-releasing factors (HRF). TCTPs participate in allergic and anaphylactic reactions, which suggest their potential role as therapeutic targets. The histaminergic effect of TCTP is related to its pro-inflammatory functions. An initial characterization of LiTCTP in animal models showed that this toxin can increase the microvascular permeability of skin vessels and induce paw edema in a dose-dependent manner. We evaluated the role of LiTCTP in vitro and in vivo in the inflammatory and allergic aspects that undergo the biological responses observed in Loxoscelism, the clinical condition after an accident with Loxosceles spiders. Our results showed LiTCTP recombinant toxin (LiRecTCTP) as an essential synergistic factor for the dermonecrotic toxin actions (LiRecDT1, known as the main toxin in the pathophysiology of Loxoscelism), revealing its contribution to the exacerbated inflammatory response clinically observed in envenomated patients.

• Authors: Marianna Boia-Ferreira, Kamila G Moreno, Alana B C Basílio, Lucas P da Silva, Larissa Vuitika, Bruna Soley, Ana Carolina M Wille, Lucélia Donatti, Katia C Barbaro, Olga M Chaim, Luiza Helena Gremski, Silvio S Veiga, Andrea Senff-Ribeiro
• Journal: Cells (Cells) Vol. 8 Issue 12 (11 22 2019) ISSN: 2073-4409 [Electronic] Switzerland
• PMID: 31766608 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Biomarkers, Tumor
• Piperidines
• Spider Venoms
• Tpt1 protein, mouse
• Tpt1 protein, rat
• Tumor Protein, Translationally-Controlled 1
• loxosceles venom
• Cimetidine
• Phosphoric Diester Hydrolases
• Promethazine
• thioperamide
• Cromolyn Sodium

Topics

• Animals
• Biomarkers, Tumor (antagonists & inhibitors, genetics, immunology)
• Cimetidine (administration & dosage, pharmacology)
• Cromolyn Sodium (administration & dosage, pharmacology)
• Dose-Response Relationship, Drug
• Hypersensitivity (drug therapy, immunology)
• Inflammation (drug therapy, immunology)
• Injections, Intraperitoneal
• Injections, Intravenous
• Mast Cells (drug effects, immunology)
• Mice
• Phosphoric Diester Hydrolases (chemistry, immunology)
• Piperidines (administration & dosage, pharmacology)
• Promethazine (administration & dosage, pharmacology)
• Rabbits
• Rats
• Skin Diseases (drug therapy, immunology)
• Spider Venoms (chemistry, immunology)
• Tumor Cells, Cultured
• Tumor Protein, Translationally-Controlled 1