F-containing initiatior for ultrasensitive fluorescent detection of lung cancer DNA via atom transfer radical polymerization.

Abstract

An ultrasensitive fluorescence method for early diagnosis of lung cancer via Nafion-initiated atom transfer radical polymerization (ATRP) is reported, in this paper. In the proposed method, thiolated peptide nucleic acid (PNA) is modified to amino magnetic beads (MBs) via a cross-linking agent to specifically capture target DNA (tDNA), and the initiator (Nafion) of ATRP is attached to PNA/DNA heteroduplexes based on the phosphate groups of the tDNA and sulfonate groups of Nafion via phosphate-Zr4+-sulfonate chemistry. Nafion as a macroinitiator of ATRP possesses multiple C-F active sites to initiate polymerization, and numerous polymeric chains that significantly amplify the fluorescent signal are formed. Under optimal conditions, a good linear relationship is obtained in the range of 0.1 nM-0.1 fM with correlation coefficients of 0.9975, and the detection limit is as low as 35.5 aM (∼214 molecules). The proposed strategy has several advantages of simplicity, cost-effectiveness, selectivity and sensitivity. More importantly, the anti-interference results demonstrate that the proposed Nafion-initiated ATRP strategy has great potential in bioanalytical applications.

• Authors: Jingyu Zhang, Qianrui Liu, Yanyan Ba, Jiamin Cheng, Huaixia Yang, Ying Cui, Jinming Kong, Xueji Zhang
• Journal: Analytica chimica acta (Anal Chim Acta) Vol. 1094 Pg. 99-105 (Jan 15 2020) ISSN: 1873-4324 [Electronic] Netherlands
• PMID: 31761052 (Publication Type: Journal Article)
• Copyright: Copyright © 2019 Elsevier B.V. All rights reserved.

Chemical References

• Acrylates
• Acrylic Resins
• Circulating Tumor DNA
• Fluoresceins
• Fluorescent Dyes
• Fluorocarbon Polymers
• Peptide Nucleic Acids
• perfluorosulfonic acid

Topics

• Acrylates (chemistry)
• Acrylic Resins (chemical synthesis, chemistry)
• Circulating Tumor DNA (blood, genetics)
• Fluoresceins (chemical synthesis, chemistry)
• Fluorescence
• Fluorescent Dyes (chemical synthesis, chemistry)
• Fluorocarbon Polymers (chemistry)
• Humans
• Limit of Detection
• Lung Neoplasms (diagnosis)
• Nucleic Acid Hybridization
• Peptide Nucleic Acids (chemistry, genetics)
• Polymerization
• Spectrometry, Fluorescence (methods)