Abstract | BACKGROUND: METHODS: Differentially expressed circRNAs between ESCC and paired adjacent normal tissues were identified using microarrays. The effects of a specific differentially expressed circRNA (circGSK3β) on tumor progression were explored in vitro and in vivo. Plasma samples from patients with ESCC, benign lesions and healthy controls were subjected to droplet digital PCR (ddPCR) analyses for circGSK3β, and the detection rates of plasma circGSK3β for ESCC were investigated. RESULTS: We demonstrated that upregulated expression of circGSK3β was positively associated with advanced clinical stage and poor outcome in patients with ESCC. We further revealed that circGSK3β promoted ESCC cell migration and invasion via direct interaction with GSK3β and inhibiting GSK3β activity, providing a novel mechanism of circRNA in cancer progression. Importantly, we identified that circGSK3β expression in plasma was a biomarker for detection of ESCC and early stage of ESCC with the area under curve (AUC) of 0.782 and 0.793, respectively. CONCLUSIONS: CircGSK3β exerts critical roles in promoting ESCC metastasis and may serve as a novel therapeutic target for ESCC patients. The plasma level of circGSK3β have potential to serve as a novel diagnostic and prognostic biomarker for ESCC detection.
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Authors | Xueting Hu, Duoguang Wu, Xiaotian He, Huiying Zhao, Zhanghai He, Jiatong Lin, Kefeng Wang, Wenjian Wang, Zihao Pan, Huayue Lin, Minghui Wang |
Journal | Molecular cancer
(Mol Cancer)
Vol. 18
Issue 1
Pg. 160
(11 14 2019)
ISSN: 1476-4598 [Electronic] England |
PMID | 31722716
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- Biomarkers
- RNA, Circular
- beta Catenin
- GSK3B protein, human
- Glycogen Synthase Kinase 3 beta
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Topics |
- Adult
- Aged
- Biomarkers
- Cell Line, Tumor
- Cell Movement
- Cell Proliferation
- Cell Transformation, Neoplastic
(genetics, metabolism)
- Esophageal Squamous Cell Carcinoma
(diagnosis, genetics, metabolism, mortality)
- Female
- Fluorescent Antibody Technique
- Gene Expression
- Gene Expression Regulation, Neoplastic
- Glycogen Synthase Kinase 3 beta
(chemistry, genetics, metabolism)
- Humans
- In Situ Hybridization
- Male
- Middle Aged
- Neoplasm Metastasis
- Neoplasm Staging
- Odds Ratio
- Prognosis
- RNA, Circular
- Signal Transduction
- Structure-Activity Relationship
- beta Catenin
(chemistry, metabolism)
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