Abstract | BACKGROUND: Central nervous system ( CNS) infections remain a major public health problem in Sub-Saharan Africa, causing 15%-25% of AIDS-related deaths. With widespread availability of antiretroviral therapy (ART) and the introduction of improved diagnostics, the epidemiology of infectious meningitis is evolving. METHODS: We prospectively enrolled adults presenting with HIV-associated meningitis in Kampala and Mbarara, Uganda, from March 2015 to September 2017. Participants had a structured, stepwise diagnostic algorithm performed of blood cryptococcal antigen (CrAg), CSF CrAg, Xpert MTB/RIF for tuberculous ( TB) meningitis (TBM), Biofire multiplex polymerase chain reaction, and traditional microscopy and cultures. RESULTS: We screened 842 consecutive adults with HIV presenting with suspected meningitis: 57% men, median age 35 years, median CD4 26 cells/mcL, and 55% presented on ART. Overall, 60.5% (509/842) were diagnosed with first-episode cryptococcal meningitis and 7.4% (62/842) with second episode. Definite/probable TB meningitis was the primary diagnosis in 6.9% (58/842); 5.3% (n = 45) had microbiologically confirmed (definite) TB meningitis. An additional 7.8% (66/842) did not meet the diagnostic threshold for definite/probable TBM but received empiric TBM therapy. Bacterial and viral meningitis were diagnosed in 1.3% (11/842) and 0.7% (6/842), respectively. The adoption of a cost-effective stepwise diagnostic algorithm allowed 79% (661/842) to have a confirmed microbiological diagnosis at an average cost of $44 per person. CONCLUSIONS: Despite widespread ART availability, Cryptococcus remains the leading cause of HIV-associated meningitis. The second most common etiology was TB meningitis, treated in 14.7% overall. The increased proportion of microbiologically confirmed TBM cases reflects the impact of new improved molecular diagnostics.
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Authors | Jayne Ellis, Ananta S Bangdiwala, Fiona V Cresswell, Joshua Rhein, Edwin Nuwagira, Kenneth Ssebambulidde, Lillian Tugume, Radha Rajasingham, Sarah C Bridge, Conrad Muzoora, David B Meya, David R Boulware |
Journal | Open forum infectious diseases
(Open Forum Infect Dis)
Vol. 6
Issue 10
Pg. ofz419
(Oct 2019)
ISSN: 2328-8957 [Print] United States |
PMID | 31660375
(Publication Type: Journal Article)
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Copyright | © The Author(s) 2019. Published by Oxford University Press on behalf of Infectious Diseases Society of America. |