Association of MTHFR and RFC1 gene polymorphisms with methotrexate efficacy and toxicity in Chinese Han patients with rheumatoid arthritis.

Abstract

OBJECTIVE:

The objective was to explore the association of methylene tetrahydrofolate reductase (MTHFR) C667T and A1298C and reduced folate carrier 1 (RFC-1) A80G single nucleotide polymorphisms (SNP) with rheumatoid arthritis (RA) and efficacy and toxicity of methotrexate (MTX) treatment in Chinese Han patients in Henan, China.

METHODS:

Two hundred ninety-six patients with RA were enrolled (cases) and 120 healthy individuals served as controls. The genotypes of MTHFR C667T and A1298C SNP and RFC-1 A80G SNP were detected by restriction fragment length polymorphism-PCR and compared between cases and controls. We analyzed correlations of clinical effect, toxicity, and SNPs after 6 months of MTX treatment.

RESULTS:

We detected no significant differences in MTHFR C677T and A1298C and RFC-1 A80G SNPs between cases and controls. The RFC-1 A80G SNP differed between RA patients with good and poor efficacy after 6 months of MTX, and was an independent factor of MTX efficacy. The MTHFR C677T SNP was differently distributed in the adverse drug reaction (ADR) and non-ADR groups and was an independent factor of MTX toxicity.

CONCLUSIONS:

In Chinese Han patients with RA, the MTHFR C667T SNP may correlate with MTX toxicity, whereas the RFC-1 A80G SNP may correlate with MTX efficacy rather than toxicity.

• Authors: Shengli Wang, Shuguang Zuo, Zhigang Liu, Xinying Ji, Zhenqiang Yao, Xinchun Wang
• Journal: The Journal of international medical research (J Int Med Res) Vol. 48 Issue 2 Pg. 300060519879588 (Feb 2020) ISSN: 1473-2300 [Electronic] England
• PMID: 31617429 (Publication Type: Journal Article)

Chemical References

• Antirheumatic Agents
• MTHFR protein, human
• Methylenetetrahydrofolate Reductase (NADPH2)
• Methotrexate

Topics

• Antirheumatic Agents (adverse effects)
• Arthritis, Rheumatoid (drug therapy, genetics)
• China
• Genotype
• Humans
• Methotrexate (adverse effects)
• Methylenetetrahydrofolate Reductase (NADPH2) (genetics)
• Polymorphism, Single Nucleotide (genetics)