A collection of four integration-free iPSC lines derived from diagnosed sporadic Alzheimer's disease patients with different APOE alleles.

Abstract

Genetic polymorphism of apolipoprotein E (APOE) confers differential susceptibility to late-onset Alzheimer's disease (LOAD). The ε3 allele of APOE, the most common isoform, does not represent a risk factor for LOAD. In contrast, the ε4 allele is the strongest genetic risk factor for this disease. Here, we present the characterization of four iPSC lines generated from dermal fibroblasts of diagnosed sporadic AD patients using Sendai viral vectors encoding OCT4, SOX2, KLF4 and c-MYC. The iPSCs expressed endogenous pluripotency markers, could be differentiated into the three germ layers, maintained the original genotypes, and were free from Sendai vectors and reprogramming factors.

• Authors: Eva Díaz-Guerra, Elena P Moreno-Jiménez, Itziar de Rojas, César Rodríguez, Eva Rodríguez-Traver, Esther Arribas-González, María Orera, Isabel Hernández, Agustín Ruiz, Carlos Vicario
• Journal: Stem cell research (Stem Cell Res) Vol. 39 Pg. 101522 (08 2019) ISSN: 1876-7753 [Electronic] England
• PMID: 31401456 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
• Copyright: Copyright © 2019 The Authors. Published by Elsevier B.V. All rights reserved.

Chemical References

• Apolipoproteins E
• KLF4 protein, human
• Kruppel-Like Factor 4

Topics

• Apolipoproteins E (genetics)
• Cell Differentiation (genetics, physiology)
• Cell Line
• Embryoid Bodies (cytology)
• Genotyping Techniques (methods)
• Humans
• Immunohistochemistry
• Karyotyping
• Kruppel-Like Factor 4
• Microsatellite Repeats (genetics)
• Reverse Transcriptase Polymerase Chain Reaction
• Sendai virus (genetics)