In vivo bioluminescence imaging of labile iron pools in a murine model of sepsis with a highly selective probe.

Abstract

Iron plays an essential role in biological system. An approach for in vivo imaging of this metal ion is needed to investigate its complex contributions to physiological and pathological processes. Herein, we present a bioluminescent probe FP-1 as a powerful tool for targeting Fe2+ detection in vitro and in vivo. The turn-on sensing scheme is based on the caged strategy of luciferin-luciferase system. FP-1 not only can detect accumulations of exogenous Fe2+ in living animal, but also has the capability of monitoring labile endogenous Fe2+ levels in animal model of sepsis. Implementation of this technique provides a valuable opportunity for understanding underlying mechanisms of Fe2+ in biological processes and disease states.

• Authors: Ping Feng, Lin Ma, Feng Xu, Xueyan Gou, Lupei Du, Bowen Ke, Minyong Li
• Journal: Talanta (Talanta) Vol. 203 Pg. 29-33 (Oct 01 2019) ISSN: 1873-3573 [Electronic] Netherlands
• PMID: 31202341 (Publication Type: Journal Article)
• Copyright: Copyright © 2019 Elsevier B.V. All rights reserved.

Chemical References

• Luminescent Agents
• Pyridines
• Firefly Luciferin
• Iron
• Luciferases, Firefly

Topics

• Animals
• Disease Models, Animal
• Fireflies (enzymology)
• Firefly Luciferin (analogs & derivatives, chemical synthesis)
• Iron (analysis, metabolism)
• Limit of Detection
• Luciferases, Firefly (chemistry)
• Luminescent Agents (chemical synthesis, chemistry)
• Luminescent Measurements (methods)
• Male
• Mice, Transgenic
• Pyridines (chemical synthesis, chemistry)
• Sepsis (metabolism)