Abstract | OBJECTIVE: The 3.7 kb deletion (-α3.7) is the most common form of α+- thalassemia found in multiple populations which can be classified into three subtypes. In order not to mis-identify it, the molecular information within each population is required. We have addressed this in northeast Thai and Laos populations. METHODS: Screening for α+- thalassemia was initially done on 1192 adult Thai subjects. In addition, 77 chromosomes of Thai newborns and 26 chromosomes of Laos with -α3.7 α+- thalassemia were also examined. All subjects were screened for -α3.7 α+- thalassemia and subtyped by PCR-RFLP assay. Exact deletion breakpoint of each -α3.7 subtype was determined by DNA sequencing. α- Globin gene haplotypes were determined. RESULTS: The proportions of -α3.7 subtypes found in 216 Thai -α3.7 chromosomes were 94.9% for -α3.7I, 4.2% for α3.7II and 0.9% for -α3.7III. All 26 Laos -α3.7 chromosomes were of -α3.7I variety. At least six α- globin gene haplotypes were associated with the -α3.7I α+- thalassemia. CONCLUSION: All -α3.7 subtypes were observed among Southeast Asian population. Haplotype analysis indicated a multiple origin of this common disorder in the region. A multiplex PCR assay has been developed for simultaneous detection of all subtypes of -α3.7 α+- thalassemia as well as other α+- thalassemia found in the region including -α4.2 α+- thalassemia, Hb Constant Spring and Hb Paksé.
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Authors | Thanyaornwanya Charoenwijitkul, Kritsada Singha, Goonnapa Fucharoen, Kanokwan Sanchaisuriya, Phuthita Thepphitak, Preawwalee Wintachai, Rossarin Karnpean, Supan Fucharoen |
Journal | Clinical biochemistry
(Clin Biochem)
Vol. 71
Pg. 31-37
(Sep 2019)
ISSN: 1873-2933 [Electronic] United States |
PMID | 31199903
(Publication Type: Journal Article)
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Copyright | Copyright © 2019 The Canadian Society of Clinical Chemists. Published by Elsevier Inc. All rights reserved. |
Topics |
- Adult
- Asia, Southeastern
- Clinical Laboratory Techniques
- Female
- Founder Effect
- Haplotypes
- Humans
- Male
- Multiplex Polymerase Chain Reaction
- alpha-Thalassemia
(genetics)
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