Abstract |
Cancer-associated chromosomal translocations are reported to generate oncogenic circular RNA ( circRNA), contributing to tumorigenesis. The fusion gene SLC34A2-ROS1 (solute carrier family 34 member 2 and ROS proto-oncogene 1) plays an important role in non-small cell lung cancer (NSCLC) progression. However, whether SLC34A2-ROS1 gene can produce circRNA remains unknown. Here, we identified two novel circRNAs (F-circSR1 and F-circSR2) generated from SLC34A2-ROS1 fusion gene, while F-circSR1 has higher expression than F-circSR2. Functional studies through gain- and loss-of-function strategies showed that both F-circSRs promote cell migration in lung cancer cells, whereas they have little effect on cell proliferation. Using the minigene GFP reporter assay, we verified that the flanking complementary sequences with canonical splicing sites are essential for F-circSR biogenesis. Therefore, our findings demonstrate the oncogenic role of F-circSR in NSCLC and highlight its therapeutic potential.
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Authors | Ke Wu, Xun Liao, Youling Gong, Juan He, Jian-Kang Zhou, Shuangyan Tan, Wenchen Pu, Canhua Huang, Yu-Quan Wei, Yong Peng |
Journal | Molecular cancer
(Mol Cancer)
Vol. 18
Issue 1
Pg. 98
(05 22 2019)
ISSN: 1476-4598 [Electronic] England |
PMID | 31118036
(Publication Type: Letter, Research Support, Non-U.S. Gov't)
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Chemical References |
- MAS1 protein, human
- Oncogene Proteins, Fusion
- Proto-Oncogene Mas
- Proto-Oncogene Proteins
- RNA, Circular
- SLC34A2 protein, human
- Sodium-Phosphate Cotransporter Proteins, Type IIb
- Protein-Tyrosine Kinases
- ROS1 protein, human
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Topics |
- A549 Cells
- Carcinoma, Non-Small-Cell Lung
(genetics)
- Cell Line, Tumor
- Cell Movement
- Gene Expression Regulation, Neoplastic
- Humans
- Lung Neoplasms
(genetics)
- Oncogene Proteins, Fusion
(genetics)
- Protein-Tyrosine Kinases
(genetics)
- Proto-Oncogene Mas
- Proto-Oncogene Proteins
(genetics)
- RNA, Circular
(genetics)
- Sodium-Phosphate Cotransporter Proteins, Type IIb
(genetics)
- Translocation, Genetic
- Up-Regulation
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