Abstract |
MicroRNAs ( miRNAs) have been reported to play critical roles in the occurrence, progression, and treatment of many cardiovascular diseases. However, the molecular mechanism by which miRNA regulates target gene expression in ischemia-reperfusion (I/R) injury in acute myocardial infarction (AMI) is not entirely clear. MiR-340-5p was reported to be downregulated in acute ischemic stroke. However, it still remains unknown whether miR-340-5p is mediated in the pathogenesis process of I/R injury after AMI. In the present study, male C57BL/6 J mice and H9C2 cardiomyocytes were used as experimental models. Real-time polymerase chain reaction analysis, Western blot analysis, and the terminal deoxynucleotidyl transferase-mediated dUTP nick-end-labeling immunofluorescence staining assay were conducted to examine related indicators in the study. We confirmed that the expression of miR-340-5p is downregulated after I/R in AMI mice and hypoxia/reperfusion (H/R)-induced cardiomyocytes. miR-340-5p could inhibit apoptosis and oxidative stress in H/R-induced H9C2 cells via downregulating activator 1 (Act1). The inhibiting action of miR-340-5p on H/R-induced apoptosis and oxidative stress in cardiomyocytes was partially reversed after Act1 overexpression. Moreover, the results showed that the NF-κB pathway may be mediated in the role of miR-340-5p on H/R-induced cardiomyocyte apoptosis and oxidative stress. We demonstrated that upregulation of miR-340-5p suppresses apoptosis and oxidative stress induced by H/R in H9C2 cells by inhibiting Act1. Therapeutic strategies that target miR-340-5p, Act1, and the NF-κB pathway could be beneficial for the treatment of I/R injury after AMI.
|
Authors | Dong Li, Jian Zhou, Baoping Yang, Yan Yu |
Journal | Journal of cellular biochemistry
(J Cell Biochem)
Vol. 120
Issue 9
Pg. 14618-14627
(09 2019)
ISSN: 1097-4644 [Electronic] United States |
PMID | 30989715
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
|
Copyright | © 2019 Wiley Periodicals, Inc. |
Chemical References |
- MIRN340 microRNA, mouse
- MIRN340-1 microRNA, rat
- MicroRNAs
- NF-kappa B
- Replication Protein C
|
Topics |
- Animals
- Apoptosis
- Cell Hypoxia
- Cell Line
- Disease Models, Animal
- Down-Regulation
- Male
- Mice
- MicroRNAs
(genetics)
- Myocardial Reperfusion Injury
(genetics, metabolism)
- Myocytes, Cardiac
(cytology)
- NF-kappa B
(metabolism)
- Oxidative Stress
- Rats
- Replication Protein C
(genetics)
- Signal Transduction
|