The expression of voltage-gated sodium channels in trigeminal nerve following chronic constriction injury in rats.

Abstract

Objectives: Despite the etiology of trigeminal neuralgia has been verified by microvascular decompression as vascular compression of the trigeminal root, very few researches concerning its underlying pathogenesis has been reported in the literature. The present study focused on those voltage-gated sodium channels, which are the structural basis for generation of ectopic action potentials. Methods: The trigeminal neuralgia modeling was obtained with infraorbital nerve chronic constriction injury (ION-CCI) in rats. Two weeks postoperatively, the infraorbital nerve (TN), the trigeminal ganglion (TG), and the brain stem (BS) were removed and analyzed with a series of molecular biological techniques. Results: Western blot depicted a significant up-regulation of Nav1.3 in TN and TG but not in BS, while none of the other isoforms (Nav1.6, Nav1.7, Nav1.8, or Nav1.9) presented a statistical change. The Nav1.3 from ION-CCI group was quantified as 2.5-fold and 1.7-fold than that from sham group in TN and TG, respectively (p < .05). Immunocytochemistry showed the Nav1.3-IR from ION-CCI group accounted for 21.2 ± 2.3% versus 6.1 ± 1.2% from sham group in TN, while the Nav1.3-positive neurons from ION-CCI group accounted for 34.1 ± 3.5% versus 11.2 ± 1.8% from sham group in TG. Immunohistochemical labeling showed the Nav1.3 was co-localized with CGRP and IB4 but not with GFAP or NF-200 in TG. Conclusion: ION-CCI may give rise to an up-regulation of Nav1.3 in trigeminal nerve as well as in C-type neurons at the trigeminal ganglion. It implied that the ectopic action potential may generate from both the compressed site of the trigeminal nerve and the ganglion rather than from the trigeminal nuclei.

• Authors: Mingxing Liu, Jun Zhong, Lei Xia, Ningning Dou, Shiting Li
• Journal: The International journal of neuroscience (Int J Neurosci) Vol. 129 Issue 10 Pg. 955-962 (Oct 2019) ISSN: 1563-5279 [Electronic] England
• PMID: 30889362 (Publication Type: Journal Article)

Chemical References

• NAV1.3 Voltage-Gated Sodium Channel
• Scn3a protein, rat
• Voltage-Gated Sodium Channels

Topics

• Animals
• Constriction
• Gene Expression
• Male
• NAV1.3 Voltage-Gated Sodium Channel (biosynthesis, genetics)
• Nerve Fibers, Unmyelinated (metabolism)
• Rats
• Rats, Sprague-Dawley
• Trigeminal Ganglion (metabolism)
• Trigeminal Nerve (metabolism)
• Trigeminal Neuralgia (genetics, metabolism)
• Voltage-Gated Sodium Channels (biosynthesis, genetics)