DP71 and SERCA2 alteration in human neurons of a Duchenne muscular dystrophy patient.

Abstract

Cognitive deficit has been identified in one third of patients affected by Duchenne Muscular Dystrophy, primarily attributed to loss of the short Dp71 dystrophin, the major brain dystrophin isoform. In this study, we investigated for the first time the Dp71 and Dp71-associated proteins cellular localization and expression in human neurons obtained by differentiation from induced pluripotent stem cell line of a patient affected by cognitive impairment. We found structural and molecular alterations in both pluripotent stem cell and derived neurons, reduced Dp71 expression, and a Ca2+ cytoplasmic overload in neurons coupled with increased expression of the SERCA2 pump in the dystrophic neurons. These results suggest that the reduction of Dp71 protein in the Duchenne muscular dystrophy neurons leads to alterations in SERCA2 and to elevated cytosolic Ca2+ concentration with consequent potential disruption of the dystrophin proteins and Dp71-associated proteins.

• Authors: Simona Ruggieri, Luigi Viggiano, Tiziana Annese, Carmela Rubolino, Andrea Gerbino, Roberta De Zio, Patrizia Corsi, Roberto Tamma, Domenico Ribatti, Mariella Errede, Francesca Operto, Lucia Margari, Nicoletta Resta, Silvia Di Tommaso, Jessica Rosati, Maria Trojano, Beatrice Nico
• Journal: Stem cell research & therapy (Stem Cell Res Ther) Vol. 10 Issue 1 Pg. 29 (01 15 2019) ISSN: 1757-6512 [Electronic] England
• PMID: 30646960 (Publication Type: Case Reports, Journal Article)

Chemical References

• Dystrophin
• apo-dystrophin 1
• Sarcoplasmic Reticulum Calcium-Transporting ATPases
• ATP2A2 protein, human

Topics

• Adult
• Cell Differentiation
• Cognitive Dysfunction (genetics)
• Dystrophin (genetics, metabolism)
• Humans
• Male
• Muscular Dystrophy, Duchenne (genetics, metabolism)
• Neurons
• Sarcoplasmic Reticulum Calcium-Transporting ATPases (genetics, metabolism)
• Young Adult