Salviae miltiorrliza-
borneol Jun-Shi coupled-herbs have been widely used for treatment of
ischemia stroke.
Salvianolic acid B was the most abundant and bioactive compound of Salviae miltiorrliza and used for prevention and treatment of
cerebrovascular diseases. However, the scientific intension and compatible mechanism of
Salvianolic acid B -
borneol combination were still unknown. A metabolomics study approach based on ultra-performance liquid chromatography quadrupole time-of-flight mass spectrometry (UPLC-Q/TOF-MS) combined with a pathological study has been applied to study the metabolic disturbances of
cerebral ischemia and evaluate the efficacies of Sal B and Sal B/
borneol against
cerebral ischemia in
middle cerebral artery occlusion (MCAO) rats. The neuroprotection of Sal B and Sal B/
borneol was reversed through the evaluation of neurological deficits,
infarct volume, and neuronal apoptosis in MCAO model. The metabonomic analysis revealed that the MCAO-induced
cerebral ischemia could be ameliorated by Sal B through improving the energy metabolism,
lipids metabolism, inflammatory responses, and
oxidant stress.
Borneol could enhance the
neuroprotective effects, was associated with the increased concentration of Sal B, and attenuate the function of
sphingolipid metabolism pathway in
cerebral ischemia rats. These findings perhaps clarify the mechanism of
neuroprotective effects of treating
ischemia stroke by Sal B or Sal B/
borneol preliminarily through metabolomics and push the quality promotion and the composition of
borneol/Sal B in secondary development of prescription.