In vivo evidence for transintestinal cholesterol efflux in patients with complete common bile duct obstruction.

Abstract

BACKGROUND:

Beyond the hepatobiliary pathway, studies have demonstrated that direct transintestinal cholesterol efflux (TICE) of plasma-derived cholesterol may contribute to reverse cholesterol transport. The clinical evidence of TICE in human remains challenged because of the difficulty to discriminate the hepatobiliary and transintestinal routes in vivo.

OBJECTIVE:

To provide the first proof of concept that TICE exists in vivo in humans by demonstrating that plasma labeled cholesterol can be excreted in the feces of patients with complete bile duct obstruction.

METHODS:

Plasma, bile, and fecal cholesterol excretion was measured by mass spectrometry 24, 48, and 72 hours after intravenous injection of D7-cholesterol in two patients presenting cholangiocarcinomas with a total obstruction of their primary bile duct.

RESULTS:

No trace of bile acids was detected in the feces of the two patients. Despite this, a significant amount of plasma D7-cholesterol was quantified in the feces of the two patients 48 hours and 72 hours after the intravenous injection.

CONCLUSION:

Our data bring a direct proof that TICE is an active pathway in humans.

• Authors: François Moreau, Claire Blanchard, Christophe Perret, Laurent Flet, Frédéric Douane, Eric Frampas, Eric Mirallie, Mikael Croyal, Audrey Aguesse, Michel Krempf, Xavier Prieur, Matthieu Pichelin, Bertrand Cariou, Cédric Le May
• Journal: Journal of clinical lipidology (J Clin Lipidol) 2019 Jan - Feb Vol. 13 Issue 1 Pg. 213-217.e1 ISSN: 1933-2874 [Print] United States
• PMID: 30342919 (Publication Type: Clinical Trial, Journal Article, Research Support, Non-U.S. Gov't)
• Copyright: Copyright © 2018 National Lipid Association. Published by Elsevier Inc. All rights reserved.

Chemical References

• Cholesterol

Topics

• Aged
• Aged, 80 and over
• Bile (metabolism)
• Cholangiocarcinoma (metabolism)
• Cholestasis (metabolism)
• Cholesterol (metabolism)
• Feces (chemistry)
• Female
• Hepatobiliary Elimination
• Humans
• Intestinal Elimination
• Intestines (physiology)
• Male
• Plasma (metabolism)
• Prospective Studies