Abstract | OBJECTIVE: Recently, studies have identified that microRNAs ( miRNAs) are novel regulators for gene expression in tumor progression including breast cancer. The aim of the study is to investigate the clinical significance and underlying functions between miR-508-3p expression and triple-negative breast cancer (TNBC) development. PATIENTS AND METHODS: Quantitative Real-time PCR (QRT-PCR) was performed to determine the expression of miR-508-3p in 54 pairs of TNBC specimens and adjacent non- tumor tissues. The association between miR-508-3p expression and clinicopathological factors was assessed using x2-test. Transwell invasion assays were used to assess cell invasion ability. Luciferase reporter assay, Western blot analyses and qRT-PCR were performed to demonstrate ZEB1 was a direct target of miR-508-3p. RESULTS: We demonstrated that miR-508-3p expression was remarkably decreased in TNBC tissues and cells. Lower miR-508-3p expression significantly associated with lymph node metastasis and distant metastasis in TNBC patients (p < 0.05). Ectopic expression of miR-508-3p significantly suppressed cell invasion ability of TNBC. MiR-508-3p overexpression suppressed cell epithelial-mesenchymal transition (EMT) phenomenon of TNBC by upregulating E-cadherin expression, but downregulating Vimentin expression. In addition, we revealed that ZEB1 was a direct target of miR-508-3p in TNBC cells. MiR-508-3p significantly suppressed cell EMT process by regulating ZEB1 expression. CONCLUSIONS: We found that miR-508-3p may be a potential therapeutic target of TNBC.
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Authors | S-J Guo, H-X Zeng, P Huang, S Wang, C-H Xie, S-J Li |
Journal | European review for medical and pharmacological sciences
(Eur Rev Med Pharmacol Sci)
Vol. 22
Issue 19
Pg. 6379-6385
(10 2018)
ISSN: 2284-0729 [Electronic] Italy |
PMID | 30338806
(Publication Type: Journal Article)
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Chemical References |
- Antigens, CD
- CDH1 protein, human
- Cadherins
- MIRN508 microRNA, human
- MicroRNAs
- VIM protein, human
- Vimentin
- ZEB1 protein, human
- Zinc Finger E-box-Binding Homeobox 1
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Topics |
- Adult
- Antigens, CD
(genetics, metabolism)
- Cadherins
(genetics, metabolism)
- Cell Line, Tumor
- Cell Movement
- Epithelial-Mesenchymal Transition
- Female
- Gene Expression Regulation, Neoplastic
- Humans
- Lymphatic Metastasis
- MicroRNAs
(genetics, metabolism)
- Middle Aged
- Neoplasm Invasiveness
- Signal Transduction
- Triple Negative Breast Neoplasms
(genetics, metabolism, pathology)
- Vimentin
(genetics, metabolism)
- Zinc Finger E-box-Binding Homeobox 1
(genetics, metabolism)
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