Evaluation of dexamethasone treated mesenchymal stem cells for recovery in neurotmesis model of peripheral nerve injury.

Abstract

OBJECTIVE:

Peripheral nerve injuries comprise significant portion of the nervous system injuries. Although peripheral nerves show some capacity of regeneration after injury, the extent of regeneration is not remarkable. The present study aimes to evaluate the regeneration of transected sciatic nerve by a therapeutic value of dexamethasone (DEX) associated with cell therapy (Cell) and biodegradable membrane (Mem) in rat.

METHODS:

Male Wistar rats (n = 42, 180-200g) were randomly divided into control (Ctrl), Membrane+ Cell, Mem, DEX, DEX+ Cell, DEX+ Mem and DEX+ Cell+ Mem groups. Functional recovery was evaluated at 2, 4, 6, 8 and 12 weeks after surgery using sciatic functional index (SFI), withdrawal reflex latency (WRL) test, electrophysiological and histological analyses.

RESULTS:

The rats in the DEX+ Cell+ Mem-treated group showed a significant improvement in SFI, WRL and electrophysiological findings during the 2nd to 12th weeks after surgery. In addition, histomorphological findings showed a significant improvement in the DEX+ Cell+ Memtreated group, at 12 weeks after surgery.

DISCUSSION:

Taken together, use of DEX associated with cell and biodegradable membrane could improve functional and histomorphological properties of the sciatic nerve after injury.

• Authors: Mehrnaz Moattari, Farahnaz Moattari, Gholamreza Kaka, Homa Mohseni Kouchesfehani, Seyed Homayoon Sadraie, Majid Naghdi, Korosh Mansouri
• Journal: Neurological research (Neurol Res) Vol. 40 Issue 12 Pg. 1060-1070 (Dec 2018) ISSN: 1743-1328 [Electronic] England
• PMID: 30246623 (Publication Type: Journal Article)

Chemical References

• Anti-Inflammatory Agents
• Antigens, CD
• Dexamethasone

Topics

• Action Potentials (physiology)
• Analysis of Variance
• Animals
• Anti-Inflammatory Agents (therapeutic use)
• Antigens, CD (metabolism)
• Dexamethasone (pharmacology)
• Disease Models, Animal
• Electromyography
• Male
• Mesenchymal Stem Cell Transplantation (methods)
• Mesenchymal Stem Cells (drug effects, physiology)
• Nerve Regeneration (drug effects)
• Rats
• Rats, Wistar
• Reaction Time (physiology)
• Recovery of Function (drug effects, physiology)
• Sciatic Neuropathy (therapy)
• Time Factors