Abstract |
Pre-existing anti-adenovirus (Ad) neutralizing antibodies (AdNAbs) are a major barrier in clinical gene therapy using Ad vectors and oncolytic Ads; however, it has not been fully elucidated which Ad capsid protein-specific antibodies are involved in AdNAb-mediated inhibition of Ad infection in vivo. In this study, mice possessing antibodies specific for each Ad capsid protein were prepared by intramuscular electroporation of each Ad capsid protein-expressing plasmid. Ad vector-mediated hepatic transduction was efficiently inhibited by more than 100-fold in mice immunized with a fiber protein-expressing plasmid or a penton base-expressing plasmid. An Ad vector pre-coated with FX before administration mediated more than 100-fold lower transduction efficiencies in the liver of warfarinized mice immunized with a fiber protein-expressing plasmid or a penton base-expressing plasmid, compared with those in the liver of warfarinized non-immunized mice. These data suggest that anti-fiber protein and anti-penton base antibodies bind to an Ad vector even though FX has already bound to the hexon, and inhibit Ad vector-mediated transduction. This study provides important clues for the development of a novel Ad vector that can circumvent inhibition with AdNAbs.
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Authors | Kyoko Tomita, Fuminori Sakurai, Shunsuke Iizuka, Masahisa Hemmi, Keisaku Wakabayashi, Mitsuhiro Machitani, Masashi Tachibana, Kazufumi Katayama, Haruhiko Kamada, Hiroyuki Mizuguchi |
Journal | Scientific reports
(Sci Rep)
Vol. 8
Issue 1
Pg. 12315
(08 17 2018)
ISSN: 2045-2322 [Electronic] England |
PMID | 30120324
(Publication Type: Journal Article)
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Chemical References |
- Antibodies, Viral
- Capsid Proteins
- penton protein, adenovirus
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Topics |
- Adenoviridae
(genetics, immunology)
- Animals
- Antibodies, Viral
(immunology)
- Capsid Proteins
(immunology)
- Female
- Gene Dosage
(genetics)
- Genetic Vectors
(genetics)
- Liver
(metabolism)
- Mice
- Mice, Inbred C57BL
- Plasmids
(genetics)
- Transduction, Genetic
(methods)
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