LncRNA UCA1 sponges miR-204-5p to promote migration, invasion and epithelial-mesenchymal transition of glioma cells via upregulation of ZEB1.

Abstract

Long non-coding RNA urothelial carcinoma associated 1 (lncRNA UCA1) promotes cancer progression and enhances chemoresistance through miR-204-5p in a few cancers. However, no studies have investigated whether UCA1 regulates glioma metastasis through miR-204-5p and its target. In the present study, cell migration, invasion and epithelial-mesenchymal transition (EMT) were evaluated in glioma cells overexpressing UCA1. The relationships among UCA1, miR-204-5p and ZEB1 were examined by real-time PCR, western blotting and dual-luciferase reporter assays. The effect of UCA1 knockdown on xenograft tumor growth was investigated. The levels of miR-204-5p, fibronectin, COL5 A1 and ZEB1 in tumor tissues were also determined. The results showed that UCA1 overexpression promoted cell migration, invasion and EMT. UCA1 interacted with miR-204-5p and decreased its level. ZEB1 was identified as a direct target of miR-204-5p and miR-204-5p negatively regulated ZEB1 expression. Moreover, UCA1 sponged miR-204-5p and partially rescued the inhibitory effect of miR-204-5p on ZEB1. In our in vivo studies, UCA1 knockdown reduced tumor volume and tumor weight. In addition, the levels of fibronectin, COL5 A1 and ZEB1 were decreased, while miR-204-5p level was increased. The present study provides the first evidence that UCA1 promotes glioma metastasis through the miR-204-5p/ZEB1 axis, contributing to the understanding of the pathogenesis of glioma.

• Authors: Chao Liang, Yang Yang, Junhong Guan, Tao Lv, Shengtao Qu, Qiang Fu, Hongyu Zhao
• Journal: Pathology, research and practice (Pathol Res Pract) Vol. 214 Issue 9 Pg. 1474-1481 (Sep 2018) ISSN: 1618-0631 [Electronic] Germany
• PMID: 30107990 (Publication Type: Journal Article)
• Copyright: Copyright © 2018. Published by Elsevier GmbH.

Chemical References

• MIRN204 microRNA, human
• MicroRNAs
• RNA, Long Noncoding
• UCA1 RNA, human
• ZEB1 protein, human
• Zinc Finger E-box-Binding Homeobox 1

Topics

• Animals
• Brain Neoplasms (genetics, pathology)
• Cell Movement (genetics)
• Epithelial-Mesenchymal Transition (genetics)
• Gene Expression Regulation, Neoplastic (genetics)
• Glioma (genetics, pathology)
• Heterografts
• Humans
• Mice
• Mice, Inbred BALB C
• Mice, Nude
• MicroRNAs (genetics)
• Neoplasm Invasiveness (genetics, pathology)
• RNA, Long Noncoding (genetics)
• Up-Regulation
• Zinc Finger E-box-Binding Homeobox 1 (biosynthesis, genetics)