Antineuroinflammatory Effect of SMTP-7 in Ischemic Mice.

Abstract

BACKGROUND:

Stachybotrys microspora triprenyl phenol-7 (SMTP-7) has both potentials of thrombolytic and neuroprotective effects, but its detailed neuroprotective mechanisms in ischemic stroke are still unclear. Here, we assessed the neuroprotective effects of SMTP-7 for anti-inflammatory and antiapoptosis mechanisms after 60 minutes of transient middle cerebral artery occlusion (tMCAO) in mice.

METHODS:

After 60minutes of tMCAO, 0.9% NaCl, tissue-type plasminogen activator (tPA), SMTP-7 or tPA+SMTP-7 was intravenously administrated through subclavian vein just before the reperfusion, and these mice were examined at 24hours after reperfusion. We histologically assessed the antineuroinflammatory effect of SMTP-7 on the expressive changes of inflammatory markers in ischemic mouse brains.

RESULTS:

Compared with the vehicle and tPA groups, SMTP-7 treatment significantly improved clinical scores and decreased the infarct volume and the numbers of TNF-α, nuclear factor-κB (NF-κB), nucleotide oligomerization domain-like receptor family pyrin domain containing 3 (NLRP3), and cleaved caspase-3-positive cells in the brain of mice at 24hours after tMCAO but not p62-positive cells. However, tPA+SMTP-7 treatment did not show such effects.

CONCLUSIONS:

The present study suggested that SMTP-7 provides a therapeutic benefit for ischemic stroke mice through anti-inflammatory and antiapoptotic effects but not antiautophagic effect.

• Authors: Yong Huang, Yasuyuki Ohta, Jingwei Shang, Ryuta Morihara, Yumiko Nakano, Yusuke Fukui, Xia Liu, Xiaowen Shi, Tian Feng, Toru Yamashita, Kota Sato, Mami Takemoto, Nozomi Hishikawa, Eriko Suzuki, Keiji Hasumi, Koji Abe
• Journal: Journal of stroke and cerebrovascular diseases : the official journal of National Stroke Association (J Stroke Cerebrovasc Dis) Vol. 27 Issue 11 Pg. 3084-3094 (Nov 2018) ISSN: 1532-8511 [Electronic] United States
• PMID: 30078758 (Publication Type: Journal Article)
• Copyright: Copyright © 2018. Published by Elsevier Inc.

Chemical References

• Anti-Inflammatory Agents
• Apoptosis Regulatory Proteins
• Benzopyrans
• Cytokines
• Inflammation Mediators
• Neuroprotective Agents
• Pyrrolidinones
• SMTP 7
• Tissue Plasminogen Activator

Topics

• Animals
• Anti-Inflammatory Agents (pharmacology)
• Apoptosis (drug effects)
• Apoptosis Regulatory Proteins (metabolism)
• Autophagy (drug effects)
• Benzopyrans (pharmacology)
• Brain (drug effects, metabolism, pathology)
• Cytokines (metabolism)
• Disease Models, Animal
• Infarction, Middle Cerebral Artery (drug therapy, metabolism, pathology)
• Inflammation Mediators (metabolism)
• Male
• Mice, Inbred ICR
• Neuroprotective Agents (pharmacology)
• Pyrrolidinones (pharmacology)
• Time Factors
• Tissue Plasminogen Activator (pharmacology)