Characterization of Epidermal Lipoxygenase Expression in Normal Human Skin and Tissue-Engineered Skin Substitutes.

Abstract

Lipoxygenases (LOXs) are enzymes likely to be involved in corneocyte lipid envelope formation and skin barrier function. In humans, mutations in epidermis-type lipoxygenase 3 ( eLOX-3) and 12R-lipoxygenase ( 12R-LOX) genes are associated with autosomal recessive congenital ichthyosis (ARCI), whereas deletion of these genes in mice causes epidermal defects. LOXs also represent a matter of interest in psoriasis as well as in cancer research. However, their expression as well as the exact role of these enzymes in normal human skin have not been fully described. Our goal was to characterize the expression of epidermal LOXs in both normal human skin and Tissue-Engineered Skin Substitutes (TESS) and to consider TESS as a potential model for LOX functional studies. Staining for epidermal differentiation markers and LOXs was performed, in parallel, on normal human skin and TESS. Our results showed similar expression profiles in TESS when compared with native skin for e-LOX3, 12R-LOX, 12S-lipoxygenase (12S-LOX), and 15-lipoxygenase 2 (15-LOX-2) but not for 15-lipoxygenase 1 (15-LOX-1). Because of their appropriate epidermal differentiation and LOX expression, TESS represent an alternative model for future studies on LOX function.

• Authors: Carolyne Simard-Bisson, Lorraine Andrée Parent, Véronique J Moulin, Bernard Fruteau de Laclos
• Journal: The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society (J Histochem Cytochem) Vol. 66 Issue 11 Pg. 813-824 (11 2018) ISSN: 1551-5044 [Electronic] United States
• PMID: 29985723 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Lipoxygenase
• Arachidonate 15-Lipoxygenase

Topics

• 3T3 Cells
• Adult
• Animals
• Arachidonate 15-Lipoxygenase (analysis)
• Cell Culture Techniques (methods)
• Cells, Cultured
• Epidermis (chemistry, enzymology, ultrastructure)
• Female
• Fibroblasts (chemistry, cytology, enzymology)
• Fluorescent Antibody Technique (methods)
• Humans
• Lipoxygenase (analysis)
• Mice
• Microscopy, Fluorescence (methods)
• Skin (chemistry, cytology, enzymology, ultrastructure)
• Skin, Artificial
• Tissue Engineering (methods)
• Young Adult