Separation and identification of phospholipids by hydrophilic interaction liquid chromatography coupled to tandem high resolution mass spectrometry with focus on isomeric phosphatidylglycerol and bis(monoacylglycero)phosphate.

Abstract

Changes in lipid composition of cells or tissue are often linked to various diseases. Studies indicate alterations of bis(monoacylglycero)phosphate (BMP) species in diseases such as cancer. Therefore, an extended phospholipid profiling method based on hydrophilic interaction liquid chromatography (HILIC) coupled to high-resolution mass spectrometry (MS) and data-dependent MS/MS acquisition was developed to separate and unambiguously identify BMP species. Lipid species identification was based on retention time, accurate mass and specific MS/MS fragments. The developed method was applied in a proof of concept study to lipid extracts of a cell culture model of conditional oncogene overexpression in MCF-7/NeuT breast cancer cells. Comparison of control and oncogene-induced MCF-7/NeuT breast cancer cells showed changes in BMP species distribution. Thereby, a shift from long-chain to shorter-chain fatty acid composition in BMP species was detected.

• Authors: Christian Vosse, Carina Wienken, Cristina Cadenas, Heiko Hayen
• Journal: Journal of chromatography. A (J Chromatogr A) Vol. 1565 Pg. 105-113 (Aug 31 2018) ISSN: 1873-3778 [Electronic] Netherlands
• PMID: 29983166 (Publication Type: Journal Article)
• Copyright: Copyright © 2018 Elsevier B.V. All rights reserved.

Chemical References

• Lysophospholipids
• Monoglycerides
• Phosphatidylglycerols
• bis(monoacylglyceryl)phosphate

Topics

• Breast Neoplasms (pathology)
• Chromatography, Liquid (methods)
• Female
• Humans
• Hydrophobic and Hydrophilic Interactions
• Isomerism
• Lysophospholipids (chemistry, isolation & purification)
• MCF-7 Cells
• Monoglycerides (chemistry, isolation & purification)
• Phosphatidylglycerols (chemistry, isolation & purification)
• Reference Standards
• Tandem Mass Spectrometry (methods)