Discrimination of Avian Influenza Virus Subtypes using Host-Cell Infection Fingerprinting by a Sulfinate-based Fluorescence Superoxide Probe.

Abstract

The current gold-standard diagnosis method for avian influenza (AI) is an embryonic egg-based hemagglutination assay followed by immunoblotting or PCR sequencing to confirm subtypes. It requires, however, specialized facilities to handle egg inoculation and incubation, and the subtyping methods relied on costly reagents. Now, the first differential sensing approach to distinguish AI subtypes is demonstrated using series of cell lines and a fluorescent sensor. Susceptibility of AI virus differs depending on genetic backgrounds of host cells. Cells were examined from different organ origins, and the infection patterns against a panel of cells were utilized for AI virus subtyping. To quantify AI infection, a highly cell-permeable fluorescent superoxide sensor was designed to visualize infection. This differential sensing strategy successfully proved discriminations of AI subtypes and demonstrated as a useful primary screening platform to monitor a large number of samples.

• Authors: Seong Cheol Hong, Dhiraj P Murale, Se-Young Jang, Md Mamunul Haque, Minah Seo, Seok Lee, Deok Ha Woo, Junghoon Kwon, Chang-Seon Song, Yun Kyung Kim, Jun-Seok Lee
• Journal: Angewandte Chemie (International ed. in English) (Angew Chem Int Ed Engl) Vol. 57 Issue 31 Pg. 9716-9721 (07 26 2018) ISSN: 1521-3773 [Electronic] Germany
• PMID: 29933516 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
• Copyright: © 2018 Wiley-VCH Verlag GmbH & Co. KGaA, Weinheim.

Chemical References

• Fluorescent Dyes
• Sulfonic Acids
• Superoxides

Topics

• Animals
• CHO Cells
• Cell Line
• Cricetulus
• Dogs
• Fluorescent Dyes (chemistry)
• Humans
• Influenza A virus (genetics)
• Madin Darby Canine Kidney Cells (virology)
• Orthomyxoviridae Infections (diagnostic imaging, genetics)
• Sulfonic Acids (chemistry)
• Superoxides (analysis, metabolism)