Three-phase solvent bar liquid-phase microextraction combined with high-performance liquid chromatography to determine sarcosine in human urine.

Abstract

Sarcosine is a potential prostate cancer marker. In this study, we developed a method of three-phase solvent bar liquid-phase microextraction combined with high-performance liquid chromatography to determine sarcosine after derivatization with 4-dimethylarminoazobenzene-4-sulfonyl chloride from human urine. The effects of different extraction conditions on extraction efficiency were investigated and optimized. Under optimum experimental conditions, a calibration graph exhibited linearity over the range of 0.05-25 μmol/L with a correlation coefficient (r2 ) of 0.9990. The enrichment factor was 168, and the detection limit was 0.02 μmol/L. The method was successfully used to analyze sarcosine in human urine and non-invasive detection, and good spiked recoveries ranging from 90.5 to 93.6% were obtained. The proposed method exhibited high sensitivity, high enrichment factor, good precision, and a simple setup. It may contribute to the early accurate diagnosis and the progression monitoring of prostatic carcinoma.

• Authors: Yuan Huang, Xiaobing Huang, Liping Huang, Qicai Liu, Yun Lei, Lijuan Yang, Liying Huang
• Journal: Journal of separation science (J Sep Sci) Vol. 41 Issue 15 Pg. 3121-3128 (Aug 2018) ISSN: 1615-9314 [Electronic] Germany
• PMID: 29878649 (Publication Type: Journal Article)
• Copyright: © 2018 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

Chemical References

• Solvents
• Sarcosine

Topics

• Calibration
• Chromatography, High Pressure Liquid
• Humans
• Liquid Phase Microextraction
• Molecular Structure
• Sarcosine (chemistry, urine)
• Solvents (chemistry)