Metal Carbonyls for the Biointerference-Free Ratiometric Surface-Enhanced Raman Spectroscopy-Based Assay for Cell-Free Circulating DNA of Epstein-Barr Virus in Blood.

Abstract

By taking advantage of the spectral properties of metal carbonyls, we have designed a surface-enhanced Raman spectroscopy (SERS) ratiometric assay for measuring cell-free circulating DNA (cfDNA) from Epstein-Barr virus in blood for nasopharyngeal carcinoma (NPC). This assay consists of a rhenium carbonyl (Re-CO) to serve as a DNA probe, an osmium carbonyl (Os-CO) embedded within the SERS-active substrate as an internal reference, and a streptavidin layer on the surface of the substrate. Hybridization of cfDNA with biotinylated-capture sequence leads to immobilization of cfDNA on the substrate. The binding of Re-CO via daunorubicin (DNR) to cfDNA is accompanied by an appearance of a strong symmetry stretching vibrations peak at 2113 cm-1, which has spectral overlap with Os-CO (2025 cm-1). This results in an increase in the I2113/ I2025 ratio and quantitatively correlates with cfDNA. This SERS assay can be readily used to detect cfDNA in blood samples from patients due to the intensity ratio of I2113/ I2025 lying in a silent region (1780-2200 cm-1) in the SERS spectrum of the biomolecules.

• Authors: Duo Lin, Tianxun Gong, Zi-Yao Hong, Sufang Qiu, Jianji Pan, Chinh-Yu Tseng, Shangyuan Feng, Rong Chen, Kien Voon Kong
• Journal: Analytical chemistry (Anal Chem) Vol. 90 Issue 12 Pg. 7139-7147 (06 19 2018) ISSN: 1520-6882 [Electronic] United States
• PMID: 29808995 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Cell-Free Nucleic Acids
• DNA, Viral
• Osmium
• Rhenium
• Carbon Monoxide

Topics

• Carbon Monoxide (chemistry)
• Cell-Free Nucleic Acids (blood, genetics)
• DNA, Viral (blood, genetics)
• Herpesvirus 4, Human (genetics, isolation & purification)
• Humans
• Osmium (chemistry)
• Real-Time Polymerase Chain Reaction
• Rhenium (chemistry)
• Spectrum Analysis, Raman
• Surface Properties